Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged

Description

This is a rat PARK2 Myc-DDK tagged ORF clone expression plasmid or lentiviral particle, ready for transfecting into mammalian cells.

Gene/Insert Name

PARK2

Species

Rat

Insert Size (bp)

1395 bp

Tag

Myc-DDK

Type

Rat Tagged ORF Clone/Lentiviral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Neomycin

Cell Selection

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGATAGTGTTTGTCAGGTTCAACTCCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAAGTGGTTGCTAAGCGACAGGGGGTTCCAGCTGACCAGCTGCGAGTGATTTTTGCTGGGAAGGAGCTGCAGAATCACCTGACAGTACAGAACTGTGACCTGGAACAACAGAGTATCGTTCACATAGTACAGAGACCACAGAGGAAAAGTCACGAAACAAATGCCTCTGGAGGGGACAAACCCCAGAGCACCCCGGAGGGCTCCATATGGGAGCCCAGAAGCTTGACTCGAGTGGACCTCAGCAGCCATATCCTGCCAGCGGACTCCGTGGGGCTGGCAGTCATTCTGGACACAGACAGCAAGAGTGACTCAGAAGCAGCCAGAGGTCCAGCAGCTAAACCCACCTACCACAGCTTTTTTGTCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTGGGAAACTCCGAGTTCAGTGCGGCACCTGCAGACAAGCAACCCTCACCTTGGCCCAGGGCCCATCTTGCTGGGATGATGTCTTAATTCCAAACCGGATGAGTGGAGAGTGCCAATCTCCAGACTGCCCTGGGACAAGAGCTGAATTTTTCTTTAAATGTGGAGCACACCCAACCTCAGACAAGGACACATCAGTAGCTTTGAACCTGATCACCAACAACAGCCGCAGCATCCCCTGCATCGCGTGCACGGATGTCAGGAACCCTGTCTTGGTCTTCCAATGTAACCACCGCCACGTGATCTGTTTGGACTGCTTCCACTTGTACTGTGTCACAAGGCTCAACGATCGGCAGTTTGTCCACGACGCTCAACTTGGCTACTCCCTGCCGTGTGTGGCTGGCTGTCCCAACTCCCTGATTAAAGAGCTCCATCACTTCAGGATCCTTGGAGAAGAGCAGTACAACAGGTACCAGCAGTATGGTGCCGAGGAGTGCGTGCTGCAGATGGGAGGTGTGCTGTGCCCCCGTCCTGGCTGCGGAGCTGGGCTGCTGCCTGAACAGGGCCAGAGGAAAGTCACCTGCGAAGGGGGCAACGGCCTGGGCTGTGGGTTCGTTTTCTGCCGGGACTGCAAGGAAGCATACCATGAAGGGGAGTGCGACTCGATGTTCGAAGCCTCGGGGGCGACTTCTCAGGCATACCGGGTGGATCAAAGAGCTGCTGAGCAAGCACGGTGGGAGGAGGCCTCCAAGGAAACCATCAAGAAAACCACCAAGCCTTGTCCTCGCTGCAATGTGCCCATTGAAAAGAATGGAGGATGTATGCACATGAAGTGTCCTCAGCCCCAGTGCAAGCTGGAGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTGAGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

ORF Nucleotide Sequence

MIVFVRFNSSYGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELQNHLTVQNCDLEQQSIVHIVQRPQRKSHETNASGGDKPQSTPEGSIWEPRSLTRVDLSSHILPADSVGLAVILDTDSKSDSEAARGPAAKPTYHSFFVYCKGPCHKVQPGKLRVQCGTCRQATLTLAQGPSCWDDVLIPNRMSGECQSPDCPGTRAEFFFKCGAHPTSDKDTSVALNLITNNSRSIPCIACTDVRNPVLVFQCNHRHVICLDCFHLYCVTRLNDRQFVHDAQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKVTCEGGNGLGCGFVFCRDCKEAYHEGECDSMFEASGATSQAYRVDQRAAEQARWEEASKETIKKTTKPCPRCNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWNRACMGDHWFDVSGAGTVAVSGTEPAWVITGLTTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Protein Sequence

SgfI-MluI

Application Notes

Ion-exchange column purified

Purification

Neural Diseases

Research Areas

Neural Signal Transduction

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

Parkin

Official Name

PRKN

Full Name

Parkin RBR E3 ubiquitin protein ligase

Alternative Names

PRKN, parkin RBR E3 ubiquitin protein ligase, AR-JP, LPRS2, PDJ, PARK2

Gene ID

5071(Human); 50873(Mouse); 56816(Rat)

Uniprot ID

O60260(Human); Q9WVS6(Mouse); Q9JK66(Rat)