Cutting Edge Models for Alzheimer's Disease Research - 5xFAD Mouse Model

Alzheimer's Disease (AD) stands as one of the most pressing health challenges of our time, affecting millions of individuals worldwide. One of the critical hurdles in understanding this complex neurodegenerative disorder is the lack of suitable animal models that faithfully recapitulate the pathological features observed in human patients. One such Alzheimer's disease mouse model that has been at the forefront of AD research is the 5xFAD mouse model.

Developed in 2006 by Oakley et al., the 5xFAD mouse model represents a significant advancement in AD research. This transgenic mouse model harbors five familial AD mutations, namely three mutations in the human amyloid precursor protein (APP) gene and two mutations in the human presenilin 1 (PS1) gene. These mutations are associated with early-onset familial AD and lead to accelerated Aβ plaque formation in the brain.

Fig. 1 Immunohistochemical staining β-amyloid in the brain of the 5XFAD mouse. There is a significant accumulation of β-amyloid plaques in a number of brain regions including the cerebral cortex (CC), hippocampus (H), thalamus (T), and amygdala (A).¹

With a wealth of research experience, Creative Biolabs can assist you in AD disease research. Besides constructing 5XFAD mouse models, we also provide other AD related research services. If you are interested in our services, please feel free to contact us for more details.

Investigative Benefits of the 5xFAD Mouse Model

5xFAD mouse model has outstanding efficacy in that it progressively develops significant hallmarks of AD, including amyloid plaques, neurofibrillary tangles, neuronal loss, and cognitive deficits. There are main advantages of 5xFAD mouse models.

• Rapid and Robust Amyloid Pathology - The 5xFAD mice develop extensive Aβ plaques at a relatively young age, with plaque deposition starting as early as two months of age.
• Reproducibility and Consistency - The 5xFAD model displays consistent and predictable disease progression. This reproducibility ensures that results obtained from different cohorts are comparable and reliable.
• Behavioral and Cognitive Phenotypes - Alongside amyloid pathology, 5xFAD mice exhibit cognitive deficits and behavioral abnormalities reminiscent of AD symptoms, including impairments in spatial memory, learning, and synaptic plasticity, which closely resemble the cognitive decline observed in human patients.
• Accessibility and Wide Usage - A large number of published studies have examined various aspects of AD using this model. This widespread usage has led to the accumulation of a vast body of literature, enabling researchers to leverage existing knowledge and build upon previous findings.

In addition to mouse models, a number of research tools are also important used in AD research. You can browse the table below to see a list of our recommended products.

Research Advances Utilizing the 5xFAD Model

The 5xFAD mouse model serves as a versatile platform for studying the efficacy and safety of potential therapies targeting various aspects of AD pathology. By utilizing the robust phenotype of the 5xFAD mouse, researchers can assess the effects of drug candidates, gene therapies, or lifestyle interventions on amyloid deposition, neuroinflammation, synaptic function, and cognitive performance.

Below are some of the advances in AD research applying the 5xFAD mouse model.

Advancements in the 5xFAD Mouse Model Technology

There have been many advancements in the 5xFAD mouse model technology in recent years, with most focusing on a better understanding of the disease's underlying mechanisms and evaluation of potential therapeutic strategies.

• Neuroscience imaging technology
  Researchers introduced novel ways of visualizing and tracking the development of amyloid plaques in the model's brain. Recent improvements in techniques like multiphoton microscopy and magnetic resonance imaging (MRI) have enabled researchers to monitor the progression of Aβ plaques and subsequent neurodegeneration in real time. Insights into the role of synaptic proteins, neurotransmitter systems, and signaling pathways in ASD pathogenesis.
• Advances in transcriptomics, proteomics, and metabolomics
  Notable progress in the realm of molecular biology, which helped enhance our knowledge of the disease's molecular mechanisms. This profound molecular profiling has been instrumental in shedding light on potential therapeutic targets.

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The 5xFAD mouse model, backed by technological advancements, has moved the scientific community several steps closer to this goal by offering unprecedented insights into the disease's progression and potential curative interventions. Future progress in the methods and tools related to this model promises to further advance our understanding of AD.

Creative Biolabs' unwavering dedication to excellence and innovation ensures that the promise of the 5xFAD model is realized.

Reference

1. DeBay, Drew R., et al. "No difference in cerebral perfusion between the wild-type and the 5XFAD mouse model of Alzheimer's disease." Scientific Reports 12.1 (2022): 22174.

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