Methods and Techniques of Neurosciences

Introduction to Neurosciences

In the last few years of studies, a growing body of neuroscience research has proven its significant role in analyzing the structure, molecular, and cell biology of the nervous system. It has become a multidisciplinary science that plays an important role in revealing the composition and behavior of the brain. To date, neuroscience has covered a wide range of areas of research, such as the molecular biology of nerve cells, the biology of the normal and abnormal brain, the molecular biology of emotional and cognitive behavior. As a result, unprecedented advances have also been made in techniques and methods based on neurosciences. Moreover, understanding the advantages and limitations of these new methods or techniques in cross-domain applications has become a research hotspot.

Methods and Techniques of Neurosciences

The speed of development of neuroscience is largely determined by the progress of its related methods and technologies. Nowadays, a wide variety of neuroscience-associated technologies have been generated for analyzing the function of the brain and nervous systems at different levels, such as animal, cellular, molecular, biochemical, single-cell, and others. In addition, a range of methods have been also developed and broadly used in many fields of neuroscience studies, including but not limited to:

Electrophysiological Methods

In recent years, electrophysiological methods have become the most popular strategies for the real-time detection of the electrical activity of neurons and the analysis of neuron-associated signaling in a variety of cell or tissue types. Typically, several electrodes of different sizes will be threaded through small holes drilled in the skull. The electrode head can touch the dura to record activity in the cerebral cortex or penetrate deep into brain tissue to record activity under a specific cortex. These methods allow data to be extracted and collected in a millisecond time frame. As a non-invasive technique, EGG has also been widely used in the diagnosis of various kinds of neurological disorders.

Imaging Methods

Currently, imaging methods have been considered as a powerful tool for studying mechanisms of action of brain development and aging. Normally, these methods can monitor brain activity without physical manipulation of the brain and thus cause no damage to the brain. Till now, imaging technologies can be classified into two main types: structural imaging and functional imaging. The former is used primarily to study the anatomical map of the brain, while the latter focuses on the biological processes of the nerves in the brain at that time. For example, in the brain imaging system, different structures of the brain, such as proteins and carbohydrates, fat, and saltwater, are visible. This information contributes to the study of brain representation in neuroscience research.

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