Primary Neuronal Cell Culture Models
Creative Biolabs has been a long-term expert in the field of neuroscience research. Based on our novel primary neuronal cell culture models, we can provide a series of products and services for our clients all over the world.
Background of Primary Neuronal Cell Culture
There are many limitations to the use of animal models for the evaluation of neurotoxins. What's more, the complexity of the nervous system has also greatly slowed the development of in vitro neural models. Composed of cells dissociated from tissues of the central nervous system (CNS) and peripheral nervous system (PNS), primary neuronal cell culture models have served as important research tools for drug discovery. Primary neuronal cell cultures are generally prepared from embryonic-stage progenitor cells, so the selection of an appropriate embryo day for preparation is critical for the optimal development of cell cultures. However, primary cultures are often only a few days to a few weeks long, so cultured adult PNS neurons are generally used in neurotoxicity studies.
Features of Primary Neuronal Cell Culture
Our primary neuronal cell culture models allow the building of complex systems as a co-culture of different cell types. Freshly prepared primary neuronal cell cultures can largely reproduce physiological conditions similar to in vivo. Compared with the continuously cultured cell lines, these cells retain their in vivo phenotype with respect to morphological, neurochemical, and electrophysiological properties. Under appropriate culture conditions, primary neuronal cells can mature and spontaneously form neuritis, thus becoming an important model for neurobiological research. It is important to note that the selection of brain regions is critical for neuroscience research, so we also provide cultured models for different brain regions.
Fig.1 Morphological alterations in neurons and microglia. (Silva, 2006)
Multiple Types of Primary Neuronal Cell Culture
- Frontal cortex culture model for studying psychosis, general toxicity, as well as neurodevelopment.
- Primary neuronal cell culture based on the cerebellum.
- Hippocampal primary neuronal cell culture for neurodegenerative disease research.
- Midbrain primary neuronal cell culture for the research of cholinergic pathway and neurodegenerative diseases.
- Spinal cord cultures with dorsal root ganglia for pain research and drug discovery.
Creative Biolabs is a leading service provider that focuses on neuroscience research. Based on our extensive experience and advanced platforms, we are confident in offering primary neural cell isolation/derivation services and neuronal differentiation services for global customers. If you are interested in our services and products, please do not hesitate to contact us for more details.
- Silva, R.F.M.; et al. Dissociated primary nerve cell cultures as models for assessment of neurotoxicity. Toxicology Letters. 2006, 163(1): 1-9.