Primary Neuronal Cell Culture Models
Primary neurons are directly obtained from animal or human nervous tissue, which are commonly used to dissect the molecular and cellular mechanisms that underlie human brain diseases. Compared with the continuously cultured cell lines, primary neurons maintain the characteristics of their tissue of origin and circumvent the selection pressure and genetic drift that occurs naturally during cell replication.
Creative Biolabs has meticulously developed a range of primary neuronal cell culture models from different nervous system regions in human, wild-type or genetically engineered mouse models, rat, and rabbit, which can be used for disease modeling, mechanism exploration, and drug development of various nervous system diseases.
Multiple Types of Primary Neuronal Cell Culture Models
- Primary culture model of mesencephalic dopaminergic (mesDA) neurons: Degeneration of mesDA neurons is the pathological hallmark of Parkinson’s disease. We offer primary culture models of central dopaminergic neurons isolated from mouse (or rat) embryos to aid in the study of the root cause and cure of this common neurodegenerative disease (NDD) in vitro.
- Primary culture model of cortical neurons: Cortical neural cell cultures are an important model system for studying neuronal development and function, neurotoxicity screening, drug discovery, and mechanisms of neurological diseases. We provide primary cortical neurons isolated from human and embryonic rodent brain tissue.
- Primary culture model of hippocampus neurons: Primary hippocampal neurons have high neuron purity, typical neuron morphology, and function, and they are very useful in vitro research on neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. We can use primary hippocampal neurons to construct models of injury, ischemia, and hypoxia, or disease to study Alzheimer's disease, encephalitis, epilepsy, ischemic stroke, and amnesia.
- Primary culture model of dorsal root ganglion (DRG) neurons: The DRG neurons are the primary neuron for sensory transmission and has emerged as an important target for the treatment of neuropathic pain (NP). We can provide primary DRG neurons isolated from lumbar DRG of embryonic or postnatal rodents for NP models.
- Primary culture model of cerebellar granule cells (CGC): CGC are generated postnatally, and constitute the most abundant and homogeneous neurons in the mammalian brain. We implement the protocol for isolating primary GGC from human and post-natal rats, making use of standard enzymatic and mechanical dissociation methods to provide an excellent model system for molecular and cell biological studies of neuronal development and function.
- Primary culture models of hypothalamic neurons: In mammals, daily patterns of sleep, appetite, and metabolism are regulated by cellular circadian clocks residing in hypothalamic neurons. We can provide primary hypothalamic neurons and transduce the cells with Bmal1/Per2 -luciferase lentivirus to observe and quantitatively describe hypothalamic neuronal circadian rhythms in real-time, which avoids labor-intensive and material-consuming biochemical experiments.
Other Types of Primary Neural Cell Culture Models
| Types | Source Species | Research Area |
|---|---|---|
| Primary culture model of microglia | mouse, rat, rabbit, human | Neuroinflammation, NDD |
| Primary culture model of astrocytes | mouse, rat, rabbit, human | Neuroinflammation, NDD |
| Primary culture model of oligodendrocytes | mouse, rat, rabbit, human | NDD, Myelination |
| Primary culture model of Schwann cells | mouse, rat, rabbit, human | Peripheral neuropathies, Myelination |
| Primary culture model of neural stem cells | mouse, rat, rabbit, human | Nervous system disease |
| Primary culture model of cerebral vascular pericytes | mouse, rat, rabbit, human | Blood-brain barrier, Neuroinflammation |
| Primary culture model of Meningeal Cells | mouse, rat, rabbit, human | Neuroimmune |
Primary Neural Cell Co-culture Models
Recently, the advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to reproduce complex neural circuits. Creative Biolabs offers a range of primary neural cell co-culture models to assist your research on neurological diseases.
Fig1. Nuclei and neurite detection in primary neuron-astrocyte co-cultures using Columbus software. (van Deijk, 2017)
Fig2. The tri-culture supports primary neurons, astrocytes, and microglia in vitro. (Goshi, 2020)
Primary Neural Cell Culture Approaches
Primary neural cell culture is one of the most important techniques in the research field of the nervous system. Creative Biolabs offers sorts of culture methods, including 2D monoculture and three-dimensional (3D) multicellular culture to go deep into the complex pathogenesis of various central nervous system and peripheral nervous system diseases.
Fig3. Schematic diagram of in vitro models of primary neurons illustrating features of each model. (Zhang, 2022)
Creative Biolabs is a leading service provider that focuses on neuroscience research. Based on our extensive experience and advanced platforms, we are confident in offering primary neuronal cell culture models assay for global customers. Meanwhile, we also provide cell products mentioned above, primary neural cell isolation/derivation services, and neuronal differentiation services.
If you are interested in our services and products, please do not hesitate to contact us for more details.
References
- van Deijk, Anne-Lieke F., et al. "High content analysis of hippocampal neuron-astrocyte co-cultures shows a positive effect of fortasyn connect on neuronal survival and postsynaptic maturation." Frontiers in Neuroscience 11 (2017): 440.
- Goshi, Noah,et al. "A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation." Journal of Neuroinflammation 17.1 (2020): 1-16.
- Zhang, Jiangang, et al. "Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective." Frontiers in Bioengineering and Biotechnology 10 (2022): 953031.
