Cell Culture Modeling Services

At the forefront of neuroscience, the use of sophisticated cell culture models is fundamental to unraveling the complexities of the brain and developing effective therapeutics. Creative Biolabs provides an extensive portfolio of cell culture services, leveraging state-of-the-art platforms to deliver physiologically relevant and highly tailored models for academic, pharmaceutical, and biotechnological research. Our commitment is to provide researchers with the essential tools needed to advance the understanding and treatment of neurological disorders.

Explore Our Cell Culture Model Services

Creative Biolabs provides a comprehensive suite of cell culture solutions, from physiologically relevant primary cells to patient-specific iPSC models. Discover how our advanced platforms can support your specific research goals.

Primary Cell Culture/Isolation Services

Capturing In Vivo Complexity

Primary cell cultures, derived directly from living tissues, offer a model system that closely mirrors the genetic and functional characteristics of their source organism. This makes them invaluable for studies requiring high physiological relevance. Our expertise includes the isolation of various neural cell types, such as neurons, microglia, astrocytes, and oligodendrocytes, from specific brain regions like the hippocampus and cortex.

We have extensive experience in creating both mono-culture and complex co-culture systems that support the growth of multiple cell types, including neurons and glia. These models allow for the investigation of intricate cell-cell interactions, which are crucial for understanding the nuanced responses of neural circuits in both healthy and diseased states. Downstream applications for these models are extensive and include protein and gene expression analysis, as well as functional assays such as live-cell imaging and electrophysiology.

Custom iPSC Generation Services

Patient-Specific Insights for Drug Discovery

Creative Biolabs harnesses the power of human induced pluripotent stem cells (hiPSCs) to create patient-specific models for disease research and drug discovery. By reprogramming somatic cells, we can generate a renewable and ethically sound source of human neural cells. This technology allows for the creation of models from patients with specific genetic backgrounds, providing unparalleled insight into disease phenotypes.

Our expertise lies in the differentiation of hiPSCs into a wide range of specific neuronal and glial cell types, including cortical neurons, motor neurons, GABAergic and glutamatergic neurons, microglia, astrocytes, and oligodendrocytes. These highly characterized cells are critical for modeling a variety of neurological disorders such as epilepsy, Alzheimer's disease, and multiple sclerosis. We utilize these cells in advanced 2D and 3D culture formats to better replicate human physiology and accelerate the development of targeted therapies.

Immortalized Cell Line Modeling Services

Scalable and Consistent Models

For research requiring high-throughput screening and robust reproducibility, immortalized cell lines are an excellent choice. These cell lines offer several advantages, including long-term viability, homogeneity, and cost-effectiveness. They are also highly amenable to genetic manipulation, making them ideal for creating stable cell lines with specific modifications to study gene function.

Our services include providing well-established immortalized neural cell lines, such as neurons, microglia, astrocytes, and oligodendrocytes. These models are instrumental in various applications, including disease modeling for neurodegenerative conditions like Alzheimer's and Parkinson's disease, large-scale drug screening, and neurodevelopmental research.

Organotypic Brain Slice Modeling Service

Specialized and Advanced Models

These 3D models are distinguished by their ability to preserve the original tissue architecture, creating an anatomically intact system that supports all CNS cell types and mimics the development of the brain in vivo. This unique system serves as a powerful bridge between dissociated cell cultures and live animal studies, allowing for sophisticated applications like long-term live imaging, repeated electrophysiological recordings, and gene transfer techniques. Ultimately, organotypic brain slice models provide a mature platform for neurotoxicology screening and testing neuroprotective molecules, advancing the preclinical assessment of novel therapeutic strategies.

Our models are prepared from postnatal or adult brains, with a focus on deriving slices from brain areas relevant to specific neurodegenerative diseases. This approach ensures the models are highly applicable for studying disease mechanisms.

Neurodegenerative Disease Modeling Services

Unlocking Disease Insights and Therapeutic Potential

Gaining insight into neurodegenerative diseases like Alzheimer's, Parkinson's, and Huntington's disease is vital for developing effective treatments. Our specialized in vitro models for neurodegenerative conditions serve as a robust platform for investigating disease mechanisms, facilitating drug discovery, and developing next-generation therapeutic strategies. We offer specific modeling for a range of conditions, including AD, PD, ALS, HD, and MS.

We utilize a variety of cell sources to create the most relevant model for your research needs:

1) Primary cells offer high physiological relevance for studying disease progression; 2) Immortalized cell lines provide consistency for high-throughput screening and mechanistic studies; 3) Human iPSC-derived cells enable personalized modeling using patient-specific genetic backgrounds. These powerful tools can be applied to study everything from Aβ toxicity in AD to α-synuclein aggregation in PD and CAG repeat dynamics in HD.

Our General Workflow

To learn more about how our cell culture models can advance your neuroscience research, please contact us today.

References

1. Puertas-Neyra, Kevin et al. "Clinical exome analysis and targeted gene repair of the c.1354dupT variant in iPSC lines from patients with PROM1-related retinopathies exhibiting diverse phenotypes." Stem Cell Res Ther. 2024;15(1):192.
2. Elfarrash, Sara et al. "Organotypic slice culture model demonstrates inter-neuronal spreading of alpha-synuclein aggregates." Acta Neuropathol Commun. 2019;7(1):213.
3. Bassil, Reina et al. "Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia." Nat Commun. 2021;12(1):5220.
4. Distributed under Open Access License CC BY 4.0. The original image was modified.