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Blood-brain Barrier Permeability: Key to Neurological Health & Disease

The blood-brain barrier (BBB) is an important physiological structure that strictly regulates the passage of substances between blood and brain parenchyma. In recent years, the study of BBB permeability has become more important due to its role in a variety of neurodegenerative diseases, stroke, multiple sclerosis, brain tumors and other neurological disorders. Creative Biolabs aims to comprehensively explore the complexity of blood-brain barrier permeability, its mechanisms, regulation, and innovative approaches for modern research.

We are well-equipped and proficient in drug development to assist our clients in their research and project development. If you are interested in our BBB-related services, please click below to learn more or feel free to contact us for more details.

Our Services Descriptions
BBB Penetration Assessment The BBB prevents the brain from absorbing the vast majority of drugs, making many otherwise promising candidates for the central nervous system less effective when they are ongoing in vivo experiments. Creative Biolabs provides stable, robust, and reproducible BBB penetration assessments for our worldwide clients, freeing you from tedious and time-consuming verification work.
BBB Transporter Assay Creative Biolabs has accumulated and explored target information and knowledge for drug delivery in the central nervous system. In addition to BBB transporter clones, we also characterize the specific information and enrichment of your transporter of interest by immunoblotting, in situ hybridization and staining techniques.
Brain Shuttle Antibody Development Service Creative Biolabs provides clients around the world with one-stop development services for brain-shuttle antibody therapies, actively delivering molecules and antibodies of your interest across the BBB through receptor-mediated cell action.

Understanding Blood Brain Barrier Permeability

The BBB consists mainly of specialized endothelial cells arranged in the capillaries of the brain. These brain endothelial cells are interconnected by tight junctions, forming a physical barrier that restricts the entry of most molecules from the bloodstream into the brain tissue. In addition, the blood-brain barrier is reinforced by pericytes, astrocyte end-feet and basement membranes, which further enhance its impermeability.

The BBB and the neurovascular unit. (Tajes, Marta, et al., 2014)Fig. 1 The BBB and the neurovascular unit.1

  • The primary responsibility of the BBB is to maintain homeostasis in the brain's microenvironment, keeping an optimal balance of ions and nutrients that effectively help neurons function. The permeability of the BBB restricts or allows the passage of certain substances between the blood and the brain. This makes it an important focus for effective drug delivery against brain lesions.
  • Factors that determine the permeability of the BBB include molecular size, charge, lipophilicity, and affinity for active transport systems. Lipophilic small molecules, gases such as oxygen and carbon dioxide, and certain polar small molecules can usually cross by passive diffusion. Water-soluble substances, on the other hand, require specialized transport systems to pass through the BBB.

We offer common BBB targeting antibody products for your research.

Cat. No Product Name Clonality Applications
NRZP-1022-ZP4049 NeuroMab™ Anti-Amyloid Beta BBB Shuttle Antibody, Clone mAb31 Monoclonal In Vitro
NRZP-1022-ZP4050 NeuroMab™ Anti-Alpha Synuclein BBB Shuttle Antibody, Clone 5C1H1 Monoclonal In Vitro
NRZP-1022-ZP4058 NeuroMab™ Anti-GDNF BBB Shuttle Antibody, CloneHIRmAb Monoclonal In Vitro
NRZP-1022-ZP4059 NeuroMab™ Anti-VCAM-1 BBB Shuttle Antibody, CloneVCAMelid Monoclonal In Vitro
NRZP-1022-ZP4061 NeuroMab™ Anti-Alpha Synuclein BBB Shuttle Antibody, Clone 10H Monoclonal In Vitro
NRZP-1022-ZP4062 NeuroMab™ Anti-EGFR BBB Shuttle Antibody, Clone 7D12 Monoclonal In Vitro
NRZP-1022-ZP4063 NeuroMab™ Anti-Amyloid Beta BBB Shuttle Antibody, Clone pa2H Monoclonal In Vitro
NRZP-1022-ZP4064 NeuroMab™ Anti-VNAR BBB Shuttle Antibody, Clone TXB2 Monoclonal In Vitro
NRZP-1022-ZP4068 NeuroMab™ Anti-JCPyVVP1 BBB Shuttle Antibody, Clone 8A7H5 Monoclonal In Vitro
NRZP-1022-ZP4069 NeuroMab™ Anti-GRIN2B BBB Shuttle Antibody, Clone Fab2 Monoclonal In Vitro
NRZP-1022-ZP4073 NeuroMab™ Anti-polyQBBB Shuttle Antibody, Clone MW1 Monoclonal In Vitro
NRZP-1022-ZP4074 NeuroMab™ Anti-Tau pSer404 BBB Shuttle Antibody, Clone 8B2 Monoclonal In Vitro
NRZP-1022-ZP4075 NeuroMab™ Anti-Tau pSer404 BBB Shuttle Antibody, Clone 6B2 Monoclonal In Vitro
NRZP-1022-ZP4076 NeuroMab™ Anti-Tau pSer404 BBB Shuttle Antibody, Clone h4E6 Monoclonal In Vitro
NRZP-1022-ZP4077 NeuroMab™ Anti-Tau oligomer BBB Shuttle Antibody, Clone M204 Monoclonal In Vitro
NRZP-1022-ZP4078 NeuroMab™ Anti-LRG1 BBB Shuttle Antibody, CloneMagacizumab Monoclonal In Vitro
NRZP-1022-ZP4079 NeuroMab™ Anti-C6 BBB Shuttle Antibody, Clone CP010 Monoclonal In Vitro
NRZP-1022-ZP4092 NeuroMab™ Anti-pTauBBB Shuttle Antibody, Clone C5.2 Monoclonal In Vitro
NRZP-1022-ZP4095 NeuroMab™ Anti-Tau pSer422 BBB Shuttle Antibody, Clone CBTAU-22.1 Monoclonal In Vitro

Mechanisms and Regulation of BBB Permeability

Certain mechanisms of BBB permeability permit the transport of essential nutrients, ions, and molecules into the brain.

Mechanisms Descriptions Permitted Substances
Transport proteins Specific transport proteins are expressed on the surface of endothelial cells, such as glucose transporter proteins (GLUT) and amino acid transporter proteins. Essential nutrients such as glucose and amino acids
Receptor-mediated transport Receptor-mediated endocytosis or transcytosis. Ligands bind to receptors on the luminal side of endothelial cells, triggering internalization and translocation to the outer lumen. A number of molecules
Passive diffusion Diffuse passively across the lipid bilayer of endothelial cells. Small lipophilic molecules, such as oxygen and carbon dioxide

The permeability of the BBB is tightly regulated to maintain CNS homeostasis and protect the brain from potential toxins. Several factors affect the integrity and permeability of the BBB.

  • Tight junction proteins: claudins, occludins.
  • Neurotransmitters and signaling molecules: inflammatory mediators such as cytokines.
  • Blood flow regulation: cerebral blood flow.

Innovative Approaches to Study Blood Brain Barrier Permeability

BBB dysfunction has been shown to be associated with a variety of neurological disorders, including neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and Amyotrophic Lateral Sclerosis (ALS), stroke, and brain tumors. Scientific advances and innovative approaches may provide new opportunities for the study and application of the BBB in neurological diseases.

  • In Vitro Models
    Over the years, researchers have developed a variety of cell-based in vitro models to mimic BBB properties and functions. The latest generation of models also incorporates multicellular complexity, integrating interactions between neurons, astrocytes and pericytes.
  • In Vivo Models
    In vivo models such as animal models (mouse, rat, zebrafish) provide an integrated biological system for studying the BBB. Techniques such as brain microdialysis and in situ cerebral perfusion have further improved the understanding of the BBB in physiologic states.
  • Molecular Imaging
    Advanced neuroscience imaging techniques such as near-infrared spectroscopy (NIRS), DCE-MRI, and light microscopy provide noninvasive means of assessing BBB permeability, bringing us closer to real-time observation and accurate measurement of the BBB environment.
  • Nanotechnology
    The use of engineered nanoparticles offers a promising approach to overcome the limitations of the BBB. They show potential for delivering therapeutic drugs to the brain, early diagnosis of diseases and monitoring disease progression.

Advances in the understanding of BBB physiology and regulation hold promise for the development of novel therapies for neurological disorders. Creative Biolabs would like to serve as your partner in advancing the emergence of new discoveries and transformative applications in neuroscience.


  1. Tajes, Marta, et al. "The blood-brain barrier: structure, function and therapeutic approaches to cross it." Molecular membrane biology 31.5 (2014): 152-167.
For Research Use Only. Not For Clinical Use.
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