Blood-brain Barrier Permeability: Key to Neurological Health & Disease
The blood-brain barrier (BBB) is an important physiological structure that strictly regulates the passage of substances between blood and brain parenchyma. In recent years, the study of BBB permeability has become more important due to its role in a variety of neurodegenerative diseases, stroke, multiple sclerosis, brain tumors and other neurological disorders. Creative Biolabs aims to comprehensively explore the complexity of blood-brain barrier permeability, its mechanisms, regulation, and innovative approaches for modern research.
We are well-equipped and proficient in drug development to assist our clients in their research and project development. If you are interested in our BBB-related services, please click below to learn more or feel free to contact us for more details.
Our Services | Descriptions |
BBB Penetration Assessment | The BBB prevents the brain from absorbing the vast majority of drugs, making many otherwise promising candidates for the central nervous system less effective when they are ongoing in vivo experiments. Creative Biolabs provides stable, robust, and reproducible BBB penetration assessments for our worldwide clients, freeing you from tedious and time-consuming verification work. |
BBB Transporter Assay | Creative Biolabs has accumulated and explored target information and knowledge for drug delivery in the central nervous system. In addition to BBB transporter clones, we also characterize the specific information and enrichment of your transporter of interest by immunoblotting, in situ hybridization and staining techniques. |
Brain Shuttle Antibody Development Service | Creative Biolabs provides clients around the world with one-stop development services for brain-shuttle antibody therapies, actively delivering molecules and antibodies of your interest across the BBB through receptor-mediated cell action. |
Understanding Blood Brain Barrier Permeability
The BBB consists mainly of specialized endothelial cells arranged in the capillaries of the brain. These brain endothelial cells are interconnected by tight junctions, forming a physical barrier that restricts the entry of most molecules from the bloodstream into the brain tissue. In addition, the blood-brain barrier is reinforced by pericytes, astrocyte end-feet and basement membranes, which further enhance its impermeability.
- The primary responsibility of the BBB is to maintain homeostasis in the brain's microenvironment, keeping an optimal balance of ions and nutrients that effectively help neurons function. The permeability of the BBB restricts or allows the passage of certain substances between the blood and the brain. This makes it an important focus for effective drug delivery against brain lesions.
- Factors that determine the permeability of the BBB include molecular size, charge, lipophilicity, and affinity for active transport systems. Lipophilic small molecules, gases such as oxygen and carbon dioxide, and certain polar small molecules can usually cross by passive diffusion. Water-soluble substances, on the other hand, require specialized transport systems to pass through the BBB.
We offer common BBB targeting antibody products for your research.
Cat. No | Product Name | Clonality | Applications |
NRZP-1022-ZP4049 | iNeuMab™ Anti-Amyloid Beta BBB Shuttle Antibody | Monoclonal | In Vitro |
NRZP-1022-ZP4050 | iNeuMab™ Anti-Alpha Synuclein BBB Shuttle Antibody (NRZP-1022-ZP4050) | Monoclonal | In Vitro |
NRZP-1022-ZP4058 | iNeuMab™ Anti-GDNF BBB Shuttle Antibody (NRZP-1022-ZP4058) | Monoclonal | In Vitro |
NRZP-1022-ZP4059 | iNeuMab™ Anti-VCAM-1 BBB Shuttle Antibody (NRZP-1022-ZP4059) | Monoclonal | In Vitro |
NRZP-1022-ZP4061 | iNeuMab™ Anti-Alpha Synuclein BBB Shuttle Antibody (NRZP-1022-ZP4061) | Monoclonal | In Vitro |
NRZP-1022-ZP4062 | iNeuMab™ Anti-EGFR BBB Shuttle Antibody (NRZP-1022-ZP4062) | Monoclonal | In Vitro |
NRZP-1022-ZP4063 | iNeuMab™ Anti-Amyloid Beta BBB Shuttle Antibody (NRZP-1022-ZP4063) | Monoclonal | In Vitro |
NRZP-1022-ZP4064 | iNeuMab™ Anti-TfR1 VNAR BBB Shuttle antibody (NRZP-1022-ZP4064) | Monoclonal | In Vitro |
NRZP-1022-ZP4068 | iNeuMab™ Anti-JCPyV VP1 BBB Shuttle Antibody (NRZP-1022-ZP4068) | Monoclonal | In Vitro |
NRZP-1022-ZP4069 | iNeuMab™ Anti-GRIN2B BBB Shuttle Antibody (NRZP-1022-ZP4069) | Monoclonal | In Vitro |
NRZP-1022-ZP4073 | iNeuMab™ Anti-polyQ BBB Shuttle Antibody (NRZP-1022-ZP4073) | Monoclonal | In Vitro |
NRZP-1022-ZP4074 | iNeuMab™ Anti-Tau pSer404 BBB Shuttle Antibody (NRZP-1022-ZP4074) | Monoclonal | In Vitro |
NRZP-1022-ZP4075 | iNeuMab™ Anti-Tau pSer404 BBB Shuttle Antibody (NRZP-1022-ZP4075) | Monoclonal | In Vitro |
NRZP-1022-ZP4076 | iNeuMab™ Anti-Tau pSer404 BBB Shuttle Antibody (NRZP-1022-ZP4076) | Monoclonal | In Vitro |
NRZP-1022-ZP4077 | iNeuMab™ Anti-Tau oligomer BBB Shuttle Antibody (NRZP-1022-ZP4077) | Monoclonal | In Vitro |
NRZP-1022-ZP4078 | iNeuMab™ Anti-LRG1 BBB Shuttle Antibody (NRZP-1022-ZP4078) | Monoclonal | In Vitro |
NRZP-1022-ZP4079 | iNeuMab™ Anti-C6 BBB Shuttle Antibody (NRZP-1022-ZP4079) | Monoclonal | In Vitro |
NRZP-1022-ZP4092 | iNeuMab™ Anti-pTau BBB Shuttle Antibody (NRZP-1022-ZP4092) | Monoclonal | In Vitro |
NRZP-1022-ZP4095 | iNeuMab™ Anti-Tau pSer422 BBB Shuttle Antibody (NRZP-1022-ZP4095) | Monoclonal | In Vitro |
Mechanisms and Regulation of BBB Permeability
Certain mechanisms of BBB permeability permit the transport of essential nutrients, ions, and molecules into the brain.
Mechanisms | Descriptions | Permitted Substances |
Transport proteins | Specific transport proteins are expressed on the surface of endothelial cells, such as glucose transporter proteins (GLUT) and amino acid transporter proteins. | Essential nutrients such as glucose and amino acids |
Receptor-mediated transport | Receptor-mediated endocytosis or transcytosis. Ligands bind to receptors on the luminal side of endothelial cells, triggering internalization and translocation to the outer lumen. | A number of molecules |
Passive diffusion | Diffuse passively across the lipid bilayer of endothelial cells. | Small lipophilic molecules, such as oxygen and carbon dioxide |
The permeability of the BBB is tightly regulated to maintain CNS homeostasis and protect the brain from potential toxins. Several factors affect the integrity and permeability of the BBB.
- Tight junction proteins: claudins, occludins.
- Neurotransmitters and signaling molecules: inflammatory mediators such as cytokines.
- Blood flow regulation: cerebral blood flow.
Innovative Approaches to Study Blood Brain Barrier Permeability
BBB dysfunction has been shown to be associated with a variety of neurological disorders, including neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and Amyotrophic Lateral Sclerosis (ALS), stroke, and brain tumors. Scientific advances and innovative approaches may provide new opportunities for the study and application of the BBB in neurological diseases.
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In Vitro Models
Over the years, researchers have developed a variety of cell-based in vitro models to mimic BBB properties and functions. The latest generation of models also incorporates multicellular complexity, integrating interactions between neurons, astrocytes and pericytes. -
In Vivo Models
In vivo models such as animal models (mouse, rat, zebrafish) provide an integrated biological system for studying the BBB. Techniques such as brain microdialysis and in situ cerebral perfusion have further improved the understanding of the BBB in physiologic states. -
Molecular Imaging
Advanced neuroscience imaging techniques such as near-infrared spectroscopy (NIRS), DCE-MRI, and light microscopy provide noninvasive means of assessing BBB permeability, bringing us closer to real-time observation and accurate measurement of the BBB environment. -
Nanotechnology
The use of engineered nanoparticles offers a promising approach to overcome the limitations of the BBB. They show potential for delivering therapeutic drugs to the brain, early diagnosis of diseases and monitoring disease progression.
Advances in the understanding of BBB physiology and regulation hold promise for the development of novel therapies for neurological disorders. Creative Biolabs would like to serve as your partner in advancing the emergence of new discoveries and transformative applications in neuroscience.
- iNeuMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- iNeuMab™ Anti-TREM2 BBB Shuttle Antibody (NRZP-1022-ZP4114) (Cat#: NRZP-1022-ZP4114)
- iNeuMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- iNeuMab™ Anti-EPHB2 Antibody (NRP-0422-P1220) (Cat#: NRP-0422-P1220)
- iNeuMab™ Anti-CD32b Antibody (NRP-0422-P1803) (Cat#: NRP-0422-P1803)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1684) (Cat#: NRP-0422-P1684)
- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- iNeuMab™ Anti-GARP Antibody (NRP-0422-P1639) (Cat#: NRP-0422-P1639)
- iNeuMab™ Anti-ApoC3 BBB Shuttle Antibody (NRZP-1022-ZP3505) (Cat#: NRZP-1022-ZP3505)
- iNeuMab™ Anti-GD2 Antibody (NRZP-1222-ZP767) (Cat#: NRZP-1222-ZP767)
- Human Brain Vascular Adventitial Fibroblasts (Cat#: NCL-21P6-014)
- iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) (Cat#: NCL-2103-P49)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Rat Immortalized Retinal Muller Cell Line rMC-1 (Cat#: NCL-2106-S93)
- Mouse Glioma Cell Line GL-261-Luc (Cat#: NCL-2108P06)
- Human Blood Brain Barrier Model (Cat#: NCL-2103-P187)
- Rat Schwann Cells RSC96, Immortalized (Cat#: NCL-2108P21)
- Mouse Microglia N9 (Cat#: NCL2110P073)
- Green Fluorescent Tau cell Line (Cat#: NCL2110P219)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- Dextran, NHS Activated (Cat#: NRZP-0722-ZP124)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-EPO BBB Shuttle Protein (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)