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Alpha-synuclein Proteins Suitable for In Vitro and In Vivo Experiments

Alpha-synuclein is a key player in complex neurodegenerative diseases, particularly Parkinson's disease (PD). The protein has received much attention for its central role in Lewy body formation. As researchers strive to unravel the mysteries of neurodegenerative diseases, alpha-synuclein has emerged as a key entity for in vitro and in vivo experiments.

Creative Biolabs describes the advantages of using alpha-synuclein in research and the various assays designed to reveal its function and dysregulation.

Structure of Alpha-Synuclein Proteins

Alpha-synuclein is a small, soluble protein that is abundantly expressed in presynaptic nerve terminals, particularly in the brain. Structurally, it is comprised of 140 amino acids and is classified into three distinct regions.

  • An amphipathic N-terminal domain: plays a crucial role in membrane interactions.
  • A hydrophobic non-amyloid beta component (NAC) region: is responsible for the aggregation of alpha-synuclein into insoluble fibrils, a characteristic feature of PD pathology.
  • An acidic C-terminal domain: is implicated in the modulation of alpha-synuclein's aggregation properties.

Antibodies are an important tool in the scientific community's ongoing efforts to obtain more information about the properties of alpha-synuclein protein and to identify diseases such as neurological disorders of the brain. Our neuroscience catalog contains a variety of alpha-synuclein monoclonal antibodies that have been validated by IHC, WB, and ICC-IF, among others.

Cat. No Product Name Clonality Applications
NAB201250LS Mouse Anti-Human α-Synuclein Phospho (Tyr39) Monoclonal Antibody (CBP3706) Monoclonal IHC-P; ELISA
NAB-08-PZ079 Rabbit Monoclonal Antibody to Alpha-synuclein (phospho S129) Monoclonal WB; DB; ELISA; IHC-P
NRP-0422-P614 NeuroMab™ Anti-Alpha Synuclein Antibody, Clone Syn-O2 Monoclonal DB; ELISA; FC; IHC; In Vitro
NAB-08-PZ735 NeuroMab™ Rabbit Anti-LRRK2 Monoclonal Antibody (CBP1887) Monoclonal ICC; IF; IHC-P; WB

Advantages of Alpha-Synuclein Proteins in Research

  • The formation of Lewy bodies is largely composed of aggregated alpha-synuclein. The relevance of alpha-synuclein to neurodegeneration positions it as a prime target for research.
  • Alpha-synuclein is integral to normal synaptic function. It regulates synaptic vesicle trafficking and neurotransmitter release.
  • Potential therapeutic target. Investigating the molecular mechanisms governing its aggregation and toxicity provides a foundation for alpha-synuclein targeting therapies aimed at modulating alpha-synuclein levels or preventing its aberrant aggregation.
  • Versatility in experimental settings. Alpha-synuclein proteins offer versatility in experimental settings, being amenable to both in vitro and in vivo studies.

Assays for Alpha-Synuclein Proteins

  • In Vitro Alpha-Synuclein Aggregation Assays
    Thioflavin T fluorescence assays and sedimentation assays are widely employed to monitor the kinetics of fibril formation. These assays enable researchers to study the impact of various factors, including pH, temperature, and the presence of co-factors, on alpha-synuclein aggregation.
  • Cell-Based Models for In Vitro Studies
    Neuroblastoma cell lines or primary neuronal culture models expressing wild-type or mutant alpha-synuclein provide insights into the cellular consequences of alpha-synuclein dysregulation. Techniques like fluorescence microscopy and immunoblotting aid in assessing protein localization, aggregation, and associated cytotoxicity.
Cat. No Product Name Types
NCL2110P208 Alpha-synuclein Stable Cell Lines Stable Cell Line
NCL2110P209 Green Fluorescent Alpha-synuclein SH-SY5Y Cell Line Stable Cell Line
NCL2110P210 Red Fluorescent Alpha-synuclein SH-SY5Y Cell Line Stable Cell Line
NCL2110P211 Green Fluorescent Alpha-synuclein HEK293 Cell Line Stable Cell Line
NCL2110P212 Red Fluorescent Alpha-synuclein HEK293 Cell Line Stable Cell Line
NCL2110P213 Green Fluorescent Alpha-synuclein U2OS Cell Line Stable Cell Line
NCL2110P229 SH-SY5Y Stable Cell Line, tau-tGFP & alpha-synuclein-TagRFP Overexpressed Overexpressed Cell
NRZP-0822-ZP3396 alpha-Synuclein Overexpression Lysate HEK293T
NCL2008ZP496 Human SNCA Knockout HEK293T Cell Line HEK293T
NCL2008ZP500 Human SNCA Knockout HEK293T Cell Lysate HEK293T
NCL2110P353] Alpha Synuclein Overexpressed RN Cell Line Overexpressed Cell
NCL2110P354 Alpha Synuclein Overexpressed SH-SY5Y Cell Line Overexpressed Cell
  • Transgenic Animal Models for In Vivo Studies
    Mouse models expressing human alpha-synuclein variants allow researchers to explore the impact of alpha-synuclein aggregation on behavior, neurodegeneration, and overall disease progression. Behavioral assays, histological analyses, and biochemical assessments in these models contribute to a comprehensive understanding of alpha-synuclein's role in neurodegeneration.
  • Alpha-Synuclein Imaging Techniques
    Advancements in imaging technologies have facilitated the visualization of alpha-synuclein in vivo.
  • Functional Assays for Synaptic Dysfunction
    Neurological electrophysiology tecchniques, such as patch-clamp recordings, paired-pulse facilitation, and neurotransmitter release assays, provide a direct measure of synaptic activity. These assays complement structural and behavioral assessments, offering a comprehensive view of alpha-synuclein's effects on neuronal communication.

As technology continues to advance, the integration of multiple experimental approaches, from in vitro aggregation assays to in vivo imaging techniques, promises a more nuanced understanding of the impact of α-synuclein on neuronal health. Furthermore, normal α-synuclein plays a crucial role in brain cell communication and is strongly associated with Parkinson's disease. Therefore, the development of customized animal models and suitable methods can help to further understand the mechanism of action (MoA) of α-syn in Parkinson's disease.

Creative Biolabs remains at the forefront of these efforts, providing innovative solutions and expertise to researchers working to decipher the complex biology of α-synuclein and its impact on human health. If you have any difficulties in the project of α-synuclein study, please contact us in time for reasonable advice and professional assistance.

For Research Use Only. Not For Clinical Use.
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