Human Astrocytes

Positive for GFAP

Species:: Human

Cell Types:: Astrocytes

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

Astrocytes constitute 20-40% of the total number of cells in the mammalian brain and they play multifaceted and vital roles in maintaining neurophysiological function of the central nervous system (CNS) by regulating homeostasis, increasing synaptic plasticity, and sustaining neuroprotective effects. Increasingly evidence suggests astrocytes play the central role in neurodegenerative disorders, stoke and other brain injury. Human astrocytes are a useful in vitro model for exploring its function and the molecular mechanisms of CNS diseases such as Alzheimer's disease and Parkinson's disease.

Primary human astrocytes from Creative Biolabs are isolated from the human brain (cerebral cortex) and can be used to study the function of CNS and how nerve cells interact.

Features

Positive for glial fibrillary acid protein (GFAP)

Cell Types

Astrocytes

Passage Number

Species

Human

Properties

Size

1M cells/vial; 5M cells/vial; 10M cells/vial

Form

Cryopreserved

Growth Pattern

Adherent

Culture Medium

It is recommended to use CocKit™ Human Astrocyte Complete Medium Kit (CAT#: NCC20-9PZ02) for culturing Primary Human astrocytes in vitro.

Coating Conditions

Poly-L-lysine (NRYF-0324-HZ22)

Shipping

Dry Ice

Storage

Liquid nitrogen

Handling Advice

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep
the cells in liquid nitrogen until they are needed for experiments.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Quality Control

Negative for mycoplasma

Donor Information

Donor Age

Fetal

Donor Health

Normal

Publications

Publications (0)

gift-card

Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The human astrocytes we ordered have been an incredible addition to our neuroscience research

The cells arrived in perfect condition, and their viability was outstanding. They responded well to the various treatments and assays we performed, showing robust and consistent results. This has significantly accelerated our project timeline.

Excellent

For our studies on astrocyte-neuron interactions

The cells were shipped quickly and in good condition. They maintained a healthy phenotype throughout our experiments, and the purity was impressive. We observed expected calcium signaling responses, which is critical for our work. Will definitely reorder for future projects.

Excellent

We purchased human astrocytes for a project on glioblastoma

They integrated well into our co-culture systems, and their proliferation rate was as advertised. The gene expression profiles matched well with primary astrocytes, giving us confidence in our experimental outcomes. Excellent product.

Excellent

I have been very pleased with the astrocytes from this supplier

They were delivered in excellent condition and have been easy to maintain in culture. The cells exhibit typical astrocytic properties, and their response to stress conditions has been consistent and reproducible.

Excellent

The astrocytes we received were of outstanding quality

We observed significant and reproducible cytokine release in response to injury models, which was crucial for our study. The technical support team was very helpful in optimizing our protocols.

Q&As

What is the recommended seeding density for culturing human astrocytes?

The recommended seeding density for human astrocytes varies depending on the experimental setup, but typically ranges from 5,000 to 10,000 cells per cm². This ensures proper cell attachment and growth. Our technical support team can provide guidance based on specific experimental requirements, including advice on scaling up cultures for larger assays or specialized applications.

What quality control measures are in place for human astrocytes?

Our human astrocytes undergo extensive quality control measures, including viability assays, morphological assessments, and mycoplasma testing. Each batch is also tested for expression of astrocytic markers like GFAP. We include a certificate of analysis with every purchase, detailing the results of these tests to ensure that researchers receive high-quality, reliable cells for their experiments.

What types of assays are compatible with human astrocytes?

Human astrocytes can be used in various assays, including immunocytochemistry, calcium imaging, gene expression analysis, and neurotoxicity assays. They are suitable for high-throughput screening and drug discovery applications. Our support team can assist in selecting the appropriate assays and provide guidance on optimizing protocols to obtain reliable and reproducible results from your experiments.