Oligodendrocyte Precursor Cell (OPC) related Assay Service

Oligodendrocyte precursor cells (OPCs) are a vital cell type in the central nervous system (CNS). They are most known for their vital role in myelination but also play a role in numerous other physiological and pathological processes, including neural development, regeneration, angiogenesis, and immune regulation. At Creative Biolabs, we offer OPC assays on primary mouse OPCs (wild-type and disease models), human primary OPCs, and human iPSC-derived OPCs. To learn more about our products and services, submit a project inquiry, or request a quote, please contact us today.

Oligodendrocyte Precursor Cells (OPCs) Products

In diseases such as multiple sclerosis (MS), Alzheimer's disease (AD), and Parkinson's disease (PD), OPC dysfunction may result in myelin degradation or interfere with remyelination. For these reasons, OPC models are invaluable for studying neurodevelopment and diseases, and are a promising source of potential targets for developing new therapeutic strategies. Creative Biolabs provides a comprehensive range of OPC products fit for any project.

Co-culture of OPCs and Neurons

Myelin regeneration is a fundamental therapeutic goal in multiple sclerosis research. Through our innovative myelination assay, we use co-cultures of OPCs and neurons to recapitulate the processes of myelination, demyelination, and remyelination in vitro. This assay allows further research and evaluation of compounds for the treatment of MS and other conditions involving myelin degradation.

Myelination Assay: Remyelination in Neuronal Co-culture

Fig.1 Co-culture neurons and OPCs using a medium that promotes myelination. On day 7, 16, and 26, MBP and TUJ1 staining images were obtained using a Zeiss LSM 980 confocal microscope, and graphs were generated with GraphPad Prism.

OPCs Assay Services

• OPC differentiation assay

OPC differentiation starts with neuroepithelial cell formation, then generation of OLIG2-expressing progenitors, formation of pre-OPCs, and finally differentiation of OPCs. We offer custom OPC identification and biomarker analysis with all common OPC markers, including OLIG2, NKX2.2, SOX10, PDGFRα, and NG2. Techniques such as immunofluorescence staining, real-time quantitative fluorescence PCR (qRT-PCR), and flow cytometry are used to identify OPCs at different stages of development.

• OPC migration assay

OPCs' migration ability plays a pivotal role in the formation and repair of myelin, as well as the development and regeneration of the nervous system. We offer a range of methods for observing and analyzing OPC migration, including live-cell imaging and electrically stimulated cell migration to measure migration efficiency and persistence. Additionally, we utilize immunofluorescence staining, qRT-PCR, flow cytometry, and other techniques to assess OPC migration ability.

• OPCs-based high-throughput drug screening assay

There have been notable advances in the implementation of high-throughput screening (HTS) technology in OPC research. These advances allow large numbers of novel compounds to be screened for treatment of myelination dysfunction. Creative Biolabs has developed an OPC cell-based HTS assay to assist clients in identifying potential clinical candidate compounds for the treatment of MS and other diseases.

At Creative Biolabs, our oligodendrocyte precursor cell assays offer reliable and precise data for your research needs. We invite you to contact us to discuss your project plans in detail.