Oligodendrocyte Precursor Cell (OPC) related Assay Service
Oligodendrocyte precursor cells (OPCs) are a vital cell type in the central nervous system (CNS). They are most known for their vital role in myelination but also play a role in numerous other physiological and pathological processes, including neural development, regeneration, angiogenesis, and immune regulation. At Creative Biolabs, we offer OPC assays on primary mouse OPCs (wild-type and disease models), human primary OPCs, and human iPSC-derived OPCs. To learn more about our products and services, submit a project inquiry, or request a quote, please contact us today.
Oligodendrocyte Precursor Cells (OPCs) Products
In diseases such as multiple sclerosis (MS), Alzheimer's disease (AD), and Parkinson's disease (PD), OPC dysfunction may result in myelin degradation or interfere with remyelination. For these reasons, OPC models are invaluable for studying neurodevelopment and diseases, and are a promising source of potential targets for developing new therapeutic strategies. Creative Biolabs provides a comprehensive range of OPC products fit for any project.
Human Oligodendrocyte Precursor Cells | Rat Oligodendrocyte Precursor Cells |
Mouse Oligodendrocyte Precursor Cells | iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) |
Co-culture of OPCs and Neurons
Myelin regeneration is a fundamental therapeutic goal in multiple sclerosis research. Through our innovative myelination assay, we use co-cultures of OPCs and neurons to recapitulate the processes of myelination, demyelination, and remyelination in vitro. This assay allows further research and evaluation of compounds for the treatment of MS and other conditions involving myelin degradation.
Myelination Assay: Remyelination in Neuronal Co-culture
Fig.1 Co-culture neurons and OPCs using a medium that promotes myelination. On day 7, 16, and 26, MBP and TUJ1 staining images were obtained using a Zeiss LSM 980 confocal microscope, and graphs were generated with GraphPad Prism.
OPCs Assay Services
- OPC differentiation assay
OPC differentiation starts with neuroepithelial cell formation, then generation of OLIG2-expressing progenitors, formation of pre-OPCs, and finally differentiation of OPCs. We offer custom OPC identification and biomarker analysis with all common OPC markers, including OLIG2, NKX2.2, SOX10, PDGFRα, and NG2. Techniques such as immunofluorescence staining, real-time quantitative fluorescence PCR (qRT-PCR), and flow cytometry are used to identify OPCs at different stages of development.
- OPC migration assay
OPCs' migration ability plays a pivotal role in the formation and repair of myelin, as well as the development and regeneration of the nervous system. We offer a range of methods for observing and analyzing OPC migration, including live-cell imaging and electrically stimulated cell migration to measure migration efficiency and persistence. Additionally, we utilize immunofluorescence staining, qRT-PCR, flow cytometry, and other techniques to assess OPC migration ability.
- OPCs-based high-throughput drug screening assay
There have been notable advances in the implementation of high-throughput screening (HTS) technology in OPC research. These advances allow large numbers of novel compounds to be screened for treatment of myelination dysfunction. Creative Biolabs has developed an OPC cell-based HTS assay to assist clients in identifying potential clinical candidate compounds for the treatment of MS and other diseases.
At Creative Biolabs, our oligodendrocyte precursor cell assays offer reliable and precise data for your research needs. We invite you to contact us to discuss your project plans in detail.
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1684) (Cat#: NRP-0422-P1684)
- iNeuMab™ Anti-ApoC3 BBB Shuttle Antibody (NRZP-1022-ZP3503) (Cat#: NRZP-1022-ZP3503)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P2275) (Cat#: NRP-0422-P2275)
- iNeuMab™ Anti-Alpha Synuclein BBB Shuttle Antibody (NRZP-1022-ZP4050) (Cat#: NRZP-1022-ZP4050)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P2293) (Cat#: NRP-0422-P2293)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1760) (Cat#: NRP-0422-P1760)
- iNeuMab™ Anti-GD2 Antibody (NRZP-1222-ZP767) (Cat#: NRZP-1222-ZP767)
- iNeuMab™ Anti-pTau Antibody (NRP-0422-P1719) (Cat#: NRP-0422-P1719)
- iNeuMab™ Anti-EPHB2 Antibody (NRP-0422-P1220) (Cat#: NRP-0422-P1220)
- iNeuMab™ Anti-TREM2 BBB Shuttle Antibody (NRZP-1022-ZP4114) (Cat#: NRZP-1022-ZP4114)
- Human Brain Vascular Adventitial Fibroblasts (Cat#: NCL-21P6-014)
- Human Glial (Oligodendrocytic) Hybrid Cell Line (MO3.13) (Cat#: NCL-2108P34)
- Human Brain Astroblastoma U-87 MG (Cat#: NCL2110P117)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- Immortalized Human Cerebral Microvascular Endothelial Cells (Cat#: NCL-2108-P020)
- Rat Muller Cell (Cat#: NCL2110P040)
- Human Blood Brain Barrier Model (Cat#: NCL-2103-P187)
- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- Mouse Microglia from C57BL/6 (Cat#: NCL-21P6-082)
- Mouse Retinal Ganglion Cell Line RGC-5 (Cat#: NCL2110P154)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- AAV2/9-hEF1a-fDIO-eNpHR 3.0-mCherry-WPRE-pA (Cat#: NTA-2012-ZP78)
- Dextran, NHS Activated (Cat#: NRZP-0722-ZP124)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- ABCA1 Antisense Oligonucleotide (NV-2106-P27) (Cat#: NV-2106-P27)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)