- NeuroMab™ Anti-FGFR1 Antibody,Clone NR3547P (Cat#: NRP-0422-P1244)
- NeuroMab™ Anti-Alpha Synuclein Antibody,Clone NR3861P (Cat#: NRP-0422-P614)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody,Clone NR1738P (Cat#: NRZP-1022-ZP3503)
- NeuroMab™ Anti-TREM2 BBB Shuttle Antibody, Clone NR19 (Cat#: NRZP-1022-ZP4114)
- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- NeuroMab™ Anti-EPHB2 Antibody,Clone NR1370P (Cat#: NRP-0422-P1220)
- NeuroMab™ Anti-GARP Antibody,Clone NR3348P (Cat#: NRP-0422-P1639)
- NeuroMab™ Anti-pTau Antibody,Clone NR3595P (Cat#: NRP-0422-P1719)
- NeuroMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- NeuroMab™ Anti-GD2 Antibody,Clone NR3007P (Cat#: NRZP-1222-ZP767)
- iNeu™ Human Motor Neurons (Cat#: NCL-2103-P71)
- iNeu™ Microglia (Cat#: NCL-7P018)
- Human Brain Vascular Pericytes (Cat#: NCL-21P6-015)
- Green Fluorescent Tau SH-SY5Y cell Line (Cat#: NCL2110P219)
- Mouse Microglia Cell Line BV-2, Immortalized (Cat#: NCL2110P153)
- Rat Olfactory Ensheathing Cells (Cat#: NRZP-1122-ZP162)
- Mouse Microglia N9 (Cat#: NCL2110P073)
- Rat Retinal Muller Cell Line, Immortalized (Cat#: NCL-21P6-192)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Mouse Glioma Cell Line GL261-GFP (Cat#: NCL-2108P04)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- pAAV-syn-jGCaMP8f-WPRE (Cat#: NTA-2106-P061)
- pAAV-syn-jGCaMP8m-WPRE (Cat#: NTA-2106-P062)
- Dextran, Cy5 Labeled, 2000 kDa (Cat#: NRZP-0722-ZP22)
- AAV2/9-hEF1a-fDIO-eNpHR 3.0-mCherry-WPRE-pA (Cat#: NTA-2012-ZP78)
- pAAV-EF1a-DIO-EGFP-WPRE (Cat#: NTA-2012AD-P285)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- pAAV-hSyn-DIO-XCaMP-R-WPRE (Cat#: NTA-2012AD-P508)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- Dextran, NHS Activated, 40 kDa (Cat#: NRZP-0722-ZP124)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, HIRMab-IDUA (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-IDS BBB Shuttle Protein, HIRMab-IDS (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, cTfRMAb-IDUA (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein, HIRMab-PON1 (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein, cTfRMAb-EPO (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, HIRMab-GDNF (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein, HIRMab-SGSH (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, cTfRMAb-TNFR (Cat#: NRZP-0423-ZP501)
Oligodendrocyte Precursor Cell (OPC) Assay
Relying on oligodendrocyte progenitor cells (OPCs) to promote remyelination offers promising prospects for the treatment of various diseases. As an expert in the field of neuroscience, Creative Biolabs provides professional OPC assay services to help global customers speed up the development of projects.
Overview of OPCs
OPC, also known as NG2-glia, is an abundant cell type in the adult central nervous system (CNS). OPCs are uniformly distributed in both gray and white matter. During CNS myelin development, OPCs proliferate and migrate to their final destination to exert their functions. Unlike some other progenitor cell populations, OPCs exist all through life. Furthermore, OPCs can regenerate oligodendrocytes (OLs) and myelin, and differentiate into mature myelinating OLs, which are closely related to the normal function of the CNS. It is worth noting that the OPC has many other functions besides serving as the ancestor of the OL. More importantly, OPCs are closely related to a variety of diseases.
Fig.1 Functions of OLs in the adult CNS. (Raffaele, et al., 2021)
The Crucial Roles of OPC Assays
Indeed, OPCs are involved in the regulation of the metabolic environment and function. In recent years, a large number of tools and methods have emerged to characterize the biology and function of OPCs, ranging from functional gene studies to single-cell sequencing. Many approaches contribute to a deeper understanding of OPCs, which in turn shed light on the pathogenesis and potential therapeutic strategies of many diseases, such as multiple sclerosis (MS) and glioma. Furthermore, OPCs are involved in the formation of gliomas, as they represent potential cell types from which these tumors originate. Therefore, OPC assays play a vital role in both disease mechanism research and drug discovery.
OPC Assay Services at Creative Biolabs
- OPC differentiation assay
Creative Biolabs offers cost-effective OLs differentiation services. With our optimized protocols, our professional team can differentiate healthy, diseased, or genetically modified induced pluripotent stem (iPS) cells into high-quality precursor OPCs and mature OPCs. Furthermore, Creative Biolabs is confident to provide additional characterization services, and our experts can even help you develop any downstream analysis you may need to advance your project.
- OPC migration assay
OPC migration is closely linked to impaired myelin remodeling in MS. Therefore, studying the migratory function of OPCs provides an attractive tool for developing new therapeutic strategies to promote myelin repair. We provide customized new OPC migration assays to our global customers.
- Drug screening
At Creative Biolabs, our dedicated scientists are focused on delivering OP-dependent drug screening services. By evaluating the effects of drug candidates on OPC proliferation, differentiation and apoptosis, we are confident in further identifying target drug molecules, which will provide a solid foundation for your follow-up research.
Fig.2 Flow scheme of OPCs-based high throughput drug screening assay. (Rosler, et al., 2016)
Promoting the differentiation and maturation of OPCs into OLs to repair myelin degeneration is an attractive strategy for multiple neurological diseases. After years of accumulation, Creative Biolabs has built a best-in-class platform and experienced team to provide customized OPC assay services to accelerate the progress of your project. Please contact us in time for more details.
References
- Raffaele, S.; et al. Oligodendrocyte dysfunction in amyotrophic lateral sclerosis: mechanisms and therapeutic perspectives. Cells. 2021, 10(3): 565.
- Afshari, F.T.; Fawcett, J.W. An in vitro assay to examine oligodendrocyte precursor cell migration on astrocytes. Astrocytes. 2012, 393-399.
- Rosler, E.S.; et al. Development of a high throughput drug screening assay to identify compounds that protect oligodendrocyte viability and differentiation under inflammatory conditions. BMC research notes. 2016, 9(1): 1-12.