Product

D3 Dopamine Receptor Internalization Assay Cell Line

[CAT#: NCL2110P291]

Applications:: GPCR Internalization

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Product Overview

Description

Cell line information: DRD3-tGFP_SH-SY5Y
Official Full Name: Dopaminergic receptor D3 (DRD3)
DNA Accesion Number: Gene Bank NM_000796
Host Cell: SH-SY5Y
Resistance: G418
Storage: Liquid Nitrogen

Features

DRD3-tGFP_SH-SY5Y contains SH-SY5Y cells stably expressing human dopaminergic receptor D3 (DRD3), labeled with tGFP protein at the N-terminus. The Creative Biolabs DRD3 redistribution cell line is designed to detect potential agonists/antagonists against DRD3, modulate the activation of dopaminergic receptor D3 and the following redistribution processes within the cell. This cell line will allow image analysis of compound-induced stimuli. The use of dopamine as a DRD3 agonist in high-content analysis (HCA) validates this highly reproducible measurement.

Applications

GPCR Internalization

Research Areas

GPCR

Assay

D3 Dopamine Receptor Internalization Assay

Assay Description

The D3 dopamine receptor internalization analysis from Creative Biolabs allows the analysis of compounds, or their ability to modulate DRD3 receptor activation and subsequent redistribution processes within the cell. When the ligand binds to DRD3, it activates the G protein, which is internalized in large, high-strength vesicles. The assay has measured fluorescence DRD3 redistribution by using image analysis algorithms by increasing the fluorescence around the nucleus. The high reproducibility of this assay allows monitoring of the D3 dopamine receptor redistribution process in high-throughput screening.

Assay Characteristics

Readout: Fluorescent Receptor Internalization

Agonist: Dopamine

EC50 Agonist: 3.90 x 10⁶-7 M for receptor internalization

Assay type: Cell-based

Detection Method: Fluorescent Redistribution

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.