Nonviral Tracers
Non-viral traditional tracers are effective tools for visualizing a large number of neuronal connections. These tracers are often injected and can be used in any animal of interest, eliminating the necessity for genetically modified mice with a specific genetic background.
Neuroanatomical tracing was first discovered by Waller, who found that damaged neuronal axons gradually degenerate from the lesion site to the distal end. Subsequently, "Waller-like" degeneration of neurons was found in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD). In the 1960s, chemical tracers were developed and became the mainstream solution for neurotracing for a long time.
Available Types
Creative Biolabs' nonviral tracers are versatile, highly sensitive, and capable of selective anterograde and retrograde labeling and have been used to study neural circuits.
Retrograde Nonviral Tracers
Chemical neurotracers include radiolabeled amino acids, which can be used to visualize and track peripheral connections in the central nervous system. 2-Deoxyglucose, which was later employed, also had substantial effects on neurotracing. Retrograde tracers can move from the axon terminals to the cell body using retrograde transport. In the following investigations, researchers used horseradish peroxidase (HRP), cholera toxin B (CTB), tetanus toxin, and hydroxyamidine (Fluoro-Gold) as retrograde tracers.
Anterograde Nonviral Tracers
Anterograde tracers move downstream of the projection via anterograde transport. For anterograde tracers, researchers have developed biotinylated dextran amines (BDAs), phytohemagglutinin L (PHA-L), and wheat germ agglutinin (WGA) for simultaneous anterograde and retrograde tracers.
Viral Tracers
Nonviral tracer systems have obvious limitations. They can only visualize the whole brain projection connections of neurons, but cannot specifically subdivide the projected cell types and synaptic connection methods. In contrast, viral tracer strategies can effectively target specific cell types, and by modifying the virus, specific small molecule tracers, such as WGA, can be effectively expressed in the target cells. As a result, viral tracing technology has gradually become a mainstream technology in neuroscience.
Creative Biolabs offers a range of anterograde and retrograde non-viral tracers. In addition, we are expanding our services to include custom adeno-associated viruses (AAVs). Please contact us to accelerate your neural tracing studies.



Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Carboxylic acid (-COOH); reacts with amino groups
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Strong tissue penetration ability; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Water soluble, low toxicity
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Strong tissue penetration ability, less affected by biological autofluorescence
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Water soluble, low toxicity
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

Low toxicity; Fluorescent labeling; Different molecular weights
- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

- Tracer Type:
- Protein Tracer; Neurotoxin
- Applications:
- Neural Tracing

- Tracer Type:
- Protein Tracer; Neurotoxin
- Applications:
- Neural Tracing

- Tracer Type:
- Protein Tracer; Neurotoxin
- Applications:
- Neural Tracing

- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing

- Tracer Type:
- Dextan Conjugate
- Applications:
- Neural Tracing
- iNeuMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody(NRZP-1022-ZP3505) (Cat#: NRZP-1022-ZP3505)
- NeuroMab™ Anti-Alpha Synuclein Antibody(NRP-0422-P614) (Cat#: NRP-0422-P614)
- NeuroMab™ Anti-CD20 Antibody(NRP-0422-P1230) (Cat#: NRP-0422-P1230)
- NeuroMab™ Anti-TNFα BBB Shuttle Antibody(NRZP-1022-ZP4105) (Cat#: NRZP-1022-ZP4105)
- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- NeuroMab™ Anti-GARP Antibody(NRP-0422-P1639) (Cat#: NRP-0422-P1639)
- NeuroMab™ Anti-FGFR1 Antibody(NRP-0422-P1244) (Cat#: NRP-0422-P1244)
- NeuroMab™ Anti-Tau Antibody(NRP-0422-P1686) (Cat#: NRP-0422-P1686)
- iNeuMab™ Mouse Anti-EFNB2 Monoclonal Antibody (CBP1159) (Cat#: NAB-0720-Z4396)
- Rat Olfactory Ensheathing Cells (Cat#: NRZP-1122-ZP162)
- Human Neurons Isolated from Cortex (Cat#: NCL-21P6-023)
- Human Astrocytes, Immortalized (Cat#: NCL-2105-P182-AM)
- Green Fluorescent BACE1 Cell Lines (Cat#: NCL2110P214)
- Mouse Glioma Cell Line GL-261-Luc (Cat#: NCL-2108P06)
- iNeu™ Human Neural Stem Cell Line (Cat#: NCL200552ZP)
- Human Brain Microvascular Endothelial Cells (Cat#: NCL-2103-P133)
- Human Brain Vascular Adventitial Fibroblasts (Cat#: NCL-21P6-014)
- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- AAV2/9-hEF1a-fDIO-eNpHR 3.0-mCherry-WPRE-pA (Cat#: NTA-2012-ZP78)
- Dextran, NHS Activated, 40 kDa (Cat#: NRZP-0722-ZP124)
- Dextran-FITC (Cat#: NTA-2011-ZP110)
- rAAV-E-SARE-Cre-ERT2-PEST-WPRE-hGH polyA (Cat#: NTA-2010-TT342)
- pAAV-syn-jGCaMP8s-WPRE (Cat#: NTA-2106-P063)
- Dextran, Cy5 Labeled, 2000 kDa (Cat#: NRZP-0722-ZP22)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-EPO BBB Shuttle Protein (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein (Cat#: NRZP-0423-ZP506)