Alzheimer's Disease (AD) Model based In Vitro Assay Services
Alzheimer's disease (AD) is an incurable disease characterized by increasing cognitive deficits. The pathology of this disease is linked to the development of amyloid plaques, which consist primarily of β-Amyloid oligomers improperly cleaved by γ-Secretase. With an aging population, AD is an increasingly pressing issue as it is the most common form of dementia. We at Creative Biolabs have created a number of cutting-edge in vitro, in vivo, and ex vivo models and platforms for use in AD research in order to aid researchers in their attempts to elucidate the mechanisms behind the disease and to find viable treatments.
Available In Vitro Models
In vitro models of AD provide a lightning-fast and direct way for revealing pathogenic alterations in AD and studying neuronal cell responses to therapies at the molecular and cellular level. Creative Biolabs' in vitro AD models have undergone considerable validation.
Fig.1 BBB microvascular network was co-cultured with neurospheres. A) A fluorescence picture of the BBB network (red) following perfusion with 40 kDa fluorescently labeled dextran (blue). B) Spatially localized 3D6-positive Aβ deposition (Cy5) in the BBB (GFP) microvascular network after 7 days of coculture with ReN-Ctrl and ReN-AD neurospheres.1
Cellular models applied to AD in vitro model
Cell models | Details |
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Induced pluripotent stem cells (iPSCs) |
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In vitro co-culture models |
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Cell Line |
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BBB model |
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Organotypic cultures |
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Methods applied to AD model in vitro
Methods | Details |
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3D culture |
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Genetic modification |
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Available AD In Vitro Services
Following model selection, we offer a variety of downstream experiments for data generation. Below is a limited list of our services most relevant to AD pathology. If you are looking for a particular experiment not listed below, please feel free to reach out to us so that we can best accommodate you.
Amyloid-beta (Amyloidβ, Aβ)
Tau
Tau Phosphorylation Assay | Tau Hyperphosporylation Assay |
Tau Aggregation Assay | Tau Uptake and Seeding Assay |
Tau Clearance Assay | Tau Degradation Assay |
Reference
- Ko, Eunkyung Clare, et al. "Accelerating the in vitro emulation of Alzheimer's disease-associated phenotypes using a novel 3D blood-brain barrier neurosphere co-culture model." Frontiers in Bioengineering and Biotechnology 11 (2023). Distributed under Open Access license CC BY 4.0, without modification.
- iNeuMab™ Anti-F-Spondin/SPON1 Antibody, Clone 3F4 (Cat#: NRZP-0822-ZP4740)
- iNeuMab™ Anti-GARP Antibody (NRP-0422-P1639) (Cat#: NRP-0422-P1639)
- iNeuMab™ Anti-CD32b Antibody (NRP-0422-P1803) (Cat#: NRP-0422-P1803)
- iNeuMab™ Rabbit Anti-LRRK2 Monoclonal Antibody (CBP1887) (Cat#: NAB-08-PZ735)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1684) (Cat#: NRP-0422-P1684)
- iNeuMab™ Anti-TREM2 Antibody (NRP-0422-P792) (Cat#: NRP-0422-P792)
- iNeuMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- iNeuMab™ Anti-ApoC3 BBB Shuttle Antibody (NRZP-1022-ZP3503) (Cat#: NRZP-1022-ZP3503)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1686) (Cat#: NRP-0422-P1686)
- iNeuMab™ Anti-pTau Antibody (NRP-0422-P1719) (Cat#: NRP-0422-P1719)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Green Fluorescent Tau cell Line (Cat#: NCL2110P219)
- Rat Retinal Muller Cell Line, Immortalized (Cat#: NCL-21P6-192)
- iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) (Cat#: NCL-2103-P49)
- Human Glial (Oligodendrocytic) Hybrid Cell Line (MO3.13) (Cat#: NCL-2108P34)
- Green Fluorescent Alpha-synuclein Cell Line (Cat#: NCL2110P209)
- iNeu™ Human Schwann Cell (Cat#: NCL-2103-P63)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Mouse Microglia from C57BL/6 (Cat#: NCL-21P6-082)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
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- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
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