Neuroinflammation Assay

Neuroinflammation can be assessed by evaluating different aspects of microglia and astrocytes in the central nervous system (CNS). Some key assays include glial activation, proinflammatory cytokine concentrations, blood-brain barrier permeability, and neuronal viability. CNS injury, brain infection, toxins, autoimmune diseases, and other conditions may all lead to the aberrant release of inflammatory factors. Neuroinflammation is a potential mechanism underlying Alzheimer's disease and other neurological disorders.

Creative Biolabs' neuroinflammation assays are efficient and accurate scientific tools that can assist researchers in understanding the biology of neurological disorders and screening prospective lead candidates.

Available Models

Creative Biolabs provides a diverse selection of neuron-glia co-culture models to meet your research needs.

Creative Biolabs can also provide custom models for your neuroinflammation research. Contact us for more information and discussion of your project.

Available Stimuli for the Induction of Neuroinflammation

Pathogen-associated molecular patterns (PAMPs) are microbial structures that elicit inflammatory responses by activating germline-encoded pattern recognition receptors (PRRs) found in both immune and non-immune cells. Receptor activation triggers intracellular inflammatory pathways, including MAPK, NF-κB, and JAK-STAT, resulting in the generation of inflammatory mediators.

Creative Biolabs provides a range of inflammation induction assay specifically tailored to your neuroinflammation research.

Downstream Assays and Evaluations of Neuroinflammation

• Detection of proinflammatory cytokines or other inflammatory markers by evaluation of protein and/or gene expression levels. We have different readouts available including traditional methods such as western blot, ELISA, qPCR but also state-of-the-art technology such as live-cell imaging, NGS and -omics approaches.
• Evaluation of changes in cell morphology, viability, migration, phagocytosis, via live-cell imaging or confocal microscopy.
• Measure changes in neuronal activity using our advanced electrophysiology platform.
• Bespoke assays.

Creative Biolabs' neuroinflammation assays are effective, sensitive, and practical means for obtaining a huge quantity of biological information with fast and precise findings. If you're looking into neuroinflammation, please contact us for service and support of the highest quality.

References

1. Luchena, Celia, et al. "A neuron, microglia, and astrocyte triple co-culture model to study Alzheimer's disease." Frontiers in Aging Neuroscience 14 (2022): 271.
2. Zheng, Yan‐Fang, et al. "A novel tri‐culture model for neuroinflammation." Journal of Neurochemistry 156.2 (2021): 249-261.