Neuroinflammation Assay Services

Neuroinflammation can be assessed by evaluating different aspects of microglia and astrocytes in the central nervous system (CNS). Some key assays include glial activation, proinflammatory cytokine concentrations, blood-brain barrier permeability, and neuronal viability. CNS injury, brain infection, toxins, autoimmune diseases, and other conditions may all lead to the aberrant release of inflammatory factors. Neuroinflammation is a potential mechanism underlying Alzheimer's disease and other neurological disorders.

Creative Biolabs' neuroinflammation assays are efficient and accurate scientific tools that can assist researchers in understanding the biology of neurological disorders and screening prospective lead candidates.

Available Models Available Stimuli Downstream Assays

Available Models

Creative Biolabs provides a diverse selection of neuron-glia co-culture models to meet your research needs.

Triple Co-Culture Model

Triple Co-Culture Model (Creative Biolabs Original)

The optimized neuron, microglia, and astrocyte triple co-culture model is a powerful tool for investigating neurodegeneration and neuroinflammation in the setting of neurodegenerative disorders.

Case Study

Fig. 1 Triple co-culture model. Fig.1 Triple co-culture model. Representative images of neurons in co-culture with microglia and astrocytes. The triple co-culture model lowered proinflammatory indicators like IL-1β and iNOS.^{1, 3}

BBB Model

Transwell culture system. (Creative Biolabs Original)

BBB dysfunction can exacerbate neuroinflammation and is an important step in the development of neuroinflammation. The transwell culture system can be used to study co-culture, cell chemotaxis, cell migration, cell invasion, and other aspects. This system also detects inflammatory mediators originating from glial cells, such as cytokines, chemokines, reactive oxygen species (ROS), and lipid mediators.

Case Study

Transwell culture system. (Creative Biolabs Original) Fig.2 BBB model. Cytokine production and the effect of exogenous cytokines.^{2, 3}

iPSCs Co-Culture System

iPSCs co-culture system. (Creative Biolabs Original)

Use iPSCs (co-culture neuron + microglia or neuro + astrocytes) in neuroinflammation models. iPSCs derived from patients enable researchers to investigate neuroinflammation processes and test potential therapeutics in a more patient-specific setting.

Creative Biolabs can also provide custom models for your neuroinflammation research. Contact us for more information and discussion of your project.

Available Stimuli for the Induction of Neuroinflammation

Pathogen-associated molecular patterns (PAMPs) are microbial structures that elicit inflammatory responses by activating germline-encoded pattern recognition receptors (PRRs) found in both immune and non-immune cells. Receptor activation triggers intracellular inflammatory pathways, including MAPK, NF-κB, and JAK-STAT, resulting in the generation of inflammatory mediators.

Creative Biolabs provides a range of inflammation induction assay specifically tailored to your neuroinflammation research.

Inflammatory Stimulation	Details
LPS	LPS activates the immune system through TLR4 and initiates a cascade of pro-inflammatory responses, making it a valuable tool for investigating inflammation.
Poly (I:C)	Poly (I:C) (polyinosinic acid cytidylic acid) is a synthetic double-stranded RNA (dsRNA) analog that is related to viral infection. TLR3 can identify poly (I:C) and activate NF-kB, resulting in the generation of cytokines.
ATP	Extracellular adenosine triphosphate (ATP) is an extracellular messenger that has a role in immune response and inflammation, and it is regarded as a danger signal molecule.
Cytokine	Cytokines activate relevant receptors, hence initiating crucial intracellular signaling pathways. Common cytokine stimulation includes: IFN-γ; IL-33; TNF-α; IL-1β and IL-6.

Downstream Assays and Evaluations of Neuroinflammation

Detection of proinflammatory cytokines or other inflammatory markers by evaluation of protein and/or gene expression levels. We have different readouts available including traditional methods such as western blot, ELISA, qPCR but also state-of-the-art technology such as live-cell imaging, NGS and -omics approaches.
Evaluation of changes in cell morphology, viability, migration, phagocytosis, via live-cell imaging or confocal microscopy.
Measure changes in neuronal activity using our advanced electrophysiology platform.
Immune cell profiling service.
Bespoke assays.

Creative Biolabs' neuroinflammation assays are effective, sensitive, and practical means for obtaining a huge quantity of biological information with fast and precise findings. If you're looking into neuroinflammation, please contact us for service and support of the highest quality.

References

Luchena, Celia, et al. "A neuron, microglia, and astrocyte triple co-culture model to study Alzheimer's disease." Frontiers in Aging Neuroscience 14 (2022): 271.
Nzou, Goodwell, et al. "Multicellular 3D neurovascular unit model for assessing hypoxia and neuroinflammation induced blood-brain barrier dysfunction." Scientific reports 10.1 (2020): 9766.
Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.

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