ASO Screening Services

Creative Biolabs is a leading biotechnology company with a strong ability to provide novel antisense oligonucleotide (ASO) screening services for our clients all over the world. Based on our advanced technologies, sophisticated equipment, and highly experienced staff, we also provide the custom protocol to ensure your satisfaction in time-saving and professional manner.

Introduction to Antisense Oligonucleotide (ASO)

Antisense oligonucleotides (ASOs) usually refer to chemically modified short-chain nucleic acids with about 15-25 nucleotides. Their sequences are complementary to the sense sequences of target mRNA according to the principle of Watson-Crick base pairing. ASOs are widely used for gene silencing and thus serve as an important tool for gene function study. In traditional drug discovery workflows, ASOs were commonly used for target identification and validation. What's more, ASOs are now being explored as therapeutics themselves. In theory, any RNA molecule in the transcriptome can be inhibited by nucleic acid-based drugs. Once inside the cell, the highly target-specific nucleic acid drugs can discriminate single nucleotide mismatches. Compared with small molecule drugs, nucleic acid drugs exhibit a faster development speed.

Fig.1 Common ASOs Mechanisms of Action. (Schoch, 2017)

Antisense Oligonucleotide (ASO) Screening Service

ASO-based therapy is an emerging field of drug development against a variety of gene-specific diseases. By using single-stranded oligonucleotides, RNA expression and splicing can be altered to reduce, restore, or modify protein expression. Through the identification of different mechanisms of action, such as RNaseH recruitment, splicing modifications, and miRNA targeting, personalized antisense therapy could be successfully predicted. Creative Biolabs can perform in vitro ASO screening using various disease cellular models, such as human primary cells and iPSC-derived neurons. For an identified RNA target, the ASO screening strategy allows the discovery of the optimal ASO.

Based on our state-of-the-art high-throughput platforms, our scientists can simultaneously test hundreds of ASOs in vitro and finally selects ASO candidates with the ability to knock down the target RNA in a dose-responsive manner. It is worth noting that ASOs therapeutics may lead to a risk of unexpected toxicity or side effects. In this case, our professional staff also provides the ASO efficacy screening service to further avoid the ASOs off-target effects of your candidate ASOs.

Creative Biolabs is a leading service provider that focuses on neuroscience research. We are confident in offering novel ASO screening services for our clients all over the world. If you are interested in our services and products, please do not hesitate to contact us for more details.

Reference

1. Schoch, K.M.; Miller, T.M. Antisense oligonucleotides: translation from mouse models to human neurodegenerative diseases. Neuron. 2017, 94(6): 1056-1070.