Product

Alpha1B Adrenoceptor Assay Cell Line

[CAT#: NCL2110P242]

Applications:: GPCR Screening

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Product Overview

Description

Cell line information: Maxensor-ADRA1B cell line
Official Full Name: Adrenoceptor alpha 1B
DNA Accession Number: EU33283
Host Cell: HEK293
Resistance: Puromycin + G618
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The Maxensor-ADRA1B contains HEK293 cells stably expressing red β-Arrestin Maxensor and green Ca2+ Maxensor biosensor and adrenergic receptor α 1B receptor (unlabeled). Creative Biolabs' Maxensor-ADRA1B cell line is designed to determine compounds or analyze their ability to modulate adrenergic receptor alpha 1B receptors. When the agonist binds to ADRA1B, the G protein is activated, which triggers a calcium-mediated cellular response and subsequent internalization mediated by β-Arrestin. This cell line has been validated by measuring calcium signaling and mobilizing β-Arrestin to analyze the intracellular distribution of Maxensor biosensors. The use of human neurotensin as an agonist in high-content analysis (HCA) and high-throughput screening (HTS) validates this highly reproducible detection method.

Applications

GPCR Screening

Application Notes

About the Maxensor biosensor series:
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.

Relevant Diseases

CNS; Parkinson's Disease

Assay

alpha1B adrenoceptor assay

Assay Description

The alpha1B Adrenoceptor Assay (ADRA1B) from Creative Biolabs provides analysis of two signaling pathways involved in receptor activation; G protein recruited through calcium flux and β-arrestin using Maxensor multiplex technology.

Assay Characteristics

Readout: Calcium Flux & β-arrestin recruitment

Agonist: Epinephrine

EC50 Agonist: 8.76 x 10⁶-8 M for Calcium assay & 1.27 x 10⁶-7 M for β-arrestin recruitment

Type of Assay: Cell based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.