Product

Human Endoneurial Endothelial Cells (THEndEC), Immortalized

[CAT#: NCL-2105-P190-AM]

These cells can be used for disease modeling, drug screening and toxicology testing.

Species:: Human

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Product Overview

Description

The immortalized human endometrial endothelial cell (THEndEC) at Creative Biolabs is a useful model that can be used to facilitate receptor-mediated in vitro BNB permeability and/or kinetic studies.

Species

Human

Properties

Freeze Medium

90% FBS+10% DMSO

Tissue Source

Brain

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Negative

Sterility Testing

The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen on dry ice

Storage

Liquid nitrogen immediately upon delivery

Research Use Only

For research use only

Quality Control

1) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells; 2) RT-PCR and western blot were used to assess the expression of CRT, GLUT-1, P-gp, LAT-1, MCT-1 and γ-GT; 3) Transendothelial electrical resistance (TEER) assessment was performed to evaluate the biophysical property of the immortalized cells; 4) Sodium fluorescein and FITC-dextran were used to evaluate the solute permeability of the immortalized cells; 5) 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI)-labelled Ac-LDL was used to evaluate Ac-LDL uptake.

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