Mouse Anti-OX40 Monoclonal Antibody (CBP2159)

Description

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. OX40 Ligand is primarily expressed on antigen presenting cells. OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells. OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells' immunosuppressive activity on effector T cells. OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease. Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease.
Human OX40 Antibody (OX.E12), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with recombinant human OX40-His (Sino Biological, 10481-H08H).

Immunogen

Recombinant human OX40-His (Sino Biological, 10481-H08H)

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse

Clone Number

CBP2159

Applications

FC; ELISA

Application Notes

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.01-0.1 µg/ml

ELISA blocking: 10-50 µg/ml

Flow cytometry: 2-10 µg/ml

Other Applications: user-optimized

Conjugation

Unconjugated

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order.)

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Handling Advice

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

OX40

Official Name

TNFRSF4

Full Name

tumor necrosis factor receptor superfamily, member 4

Alternative Names

CD134; TAX transcriptionally-activated glycoprotein 1 receptor; TXGP1L; OX40L receptor; Tumor necrosis factor receptor superfamily member 4; OX40; CD antigen CD134; IMD16; ACT35 antigen; ACT35

Gene ID

7293(Human);22163(Mouse)

Uniprot ID

P43489(Human);P47741(Mouse)