Mouse Anti-Neurofilament Heavy Polypeptide Monoclonal Antibody (CBP2434)

Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Species Reactivity

Rat; Mmammalian; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2434

Applications

IHC-P

Application Notes

IHC-P (1.0-5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Alexa Fluor 488

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NEFH

Official Name

NEFH

Full Name

Hypophosphorylated Neurofilament H

Alternative Names

Neurofilament Heavy; Neurofilament Heavy Polypeptide; Neurofilament; Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; 200 KDa Neurofilament Protein; NF-H; NFH; KIAA085; CMT2CC;

1. Siedler D, et al. 2014. Front Cell Neurosci. 8:429.