NeuroMab™ Anti-ApoE3 ApoE4 BBB Shuttle Antibody, Clone HJ153

Figure 1 depicts an Figure of a Western blot. Brain lysates from ApoE KO mice or mice expressing ApoE3, ApoE4, or murine ApoE were immunoblotted with HJ153 or HJ154. Western blot showed that HJ153 and HJ154 recognized both ApoE3 and ApoE4.

Figure 2 depicts Figures of brain tissue from 5XFAD APP transgenic mice expressing different human ApoE isoforms and stained using biotinylated HJ153 or HJ154 antibodies. This figure shows that these two antibodies stain ApoE in neurons, astrocytes, and amyloid plaques (if plaques are present). Brain tissue from ApoE KO mice was used as a negative control.

Figure 3 depicts Figures of several western blots. Use HJ152, HJ153, or HJ154 for immunoprecipitation of samples containing ApoE2, ApoE3, or ApoE4.

Figure 4 depicts graphs showing that certain anti-ApoE antibodies reduce Aβ plaques in APP/PS1-21 E4/E4 mice after ICV infusion.

Figure 5 depicts unfixed tissue sections (20 μm thick), using a biotinylated ("B") antibody.

Figure 6 shows a schematic representation of the time between 2 and 3.5 months of age with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155, HJ156.

Figure 7 shows graphs illustrating the number of Aβ plaques in APP/PS1-21 E4/E4 mice treated with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155 , HJ156 is 2 months old to 3.5 months old.

Figure 8 shows fibrous Aβ plaques measured as percent X-34 stained area in APP/PS1-21 E4/E4 mice injected weekly intraperitoneally (IP) with PBS, HJ16.6 (IgG negative control) Sedimentary chart. , and anti-ApoE antibodies HJ151, HJ155 and HJ156 from 2 to 3.5 months of age.

Figure 9 shows graphs illustrating the number of fibrous Aβ plaques in APP/PS1-21 E4/E4 mice treated with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155 Figure, and HJ156 from 2 to 3.5 months of age.

Figure 10 is a graph depicting the binding of HJ151 and HJ156 to ApoE4 in amyloid plaques in unfixed mouse brain sections and specificity for heat-induced aggregates of ApoE4.

Unfixed frozen brain sections of APPPS1/APOE4 or APPPS1/APOE KO mice were stained with anti-Aβ antibody HJ3.4, anti-ApoE antibodies HJ151 and HJ156. (Scale bar = 400 µm).

Figure 11 is a graph depicting the binding of HJ151 and HJ156 to ApoE4 in amyloid plaques in unfixed mouse brain sections and specificity for heat-induced ApoE4 aggregates.

Binding of HJ151, HJ153, and HJ156 to untreated recombinant ApoE4 (untreated) and ApoE4 that had been incubated at 40°C for 24 hours (40°C).

Figure 12 depicts the staining of microglia and plaques after acute immunization with HJ156.