NeuroMab™ Anti-GARP BBB Shuttle Antibody, Clone LHG-10.3
- Host Species:
- Humanized
- Species Reactivity:
- Human; Cynomolgus Monkey
- Applications:
- FC; Inhib; Block; In Vitro; In Vivo
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Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
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Figure 1 Cross-reactivity with macaque GARP-TGFβ measured on cells by FACS. 293E cells were transfected with human/cynomolgus GARP and human/cynomolgus TGFB. LHG-10-D and affinity-optimized variants cross-react with cynomolgus GARP-TGFB.
Figure 2 MHGARP8 and LHG-10 inhibit the suppressive activity of human Tregs in vitro.
Figure 3 MHGARP8 and LHG-10 inhibit human Treg from producing active TGF-β.
After a short in vitro amplification, human CD4+CD25hiCD127lo cells (Tregs) were re-stimulated with anti-CD3/CD28 coated beads during 24 hours, in the presence or absence of the indicated mAbs (10 μg/ml). Cells lysates were analyzed by Western Blot with antibodies against phosphorylated SMAD2 (pSMAD2), as a read-out for active TGF-β production, or β-ACTIN (loading control).
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