NeuroMab™ Anti-Netrin 1 Antibody, Clone hum03

Product Overview

Description

The antibody specific binds to the region of netrin-1 to receptor and interfering with netrin-1/receptor interaction. The antibody induces apoptosis or cell death of tumour cells expressing or overexpressing netrin-1, owing the fact that this interaction inhibits netrin-1 binding to a receptor and the multimerization of the receptor.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Humanized

Clone Number

hum03

Applications

ELISA; Block; Inhib; In Vitro; In Vivo

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Netrin 1

Official Name

NTN1

Full Name

Netrin-1

Alternative Names

NTN1; NTN1L; netrin 1; MRMV4

Gene ID

9423(Human); 18208(Mouse); 114523(Rat)

Uniprot ID

O95631(Human); O09118(Mouse); Q924Z9(Rat)

Product Pictures

In Vivo

Figure 1: In vivo effect of humanized 4C11 antibody (hum03) on the growth of transplantable osteosarcoma in rats.

Rat osteosarcoma tumors were transplanted in paratibial position after denudation of the periosteum (n=7). The rats were treated twice a week by intra-peritoneal injection of 4.4 mg/kg of humanized 4C11 hum03 or an isotypic control with or without doxorubicin (2 mg/kg) or with PBS. The figure displays the tumor fold increase at day 17.

FuncS

Figure 2: Pepscan analysis of the NET1-binding domain of the anti-Nitidine 1 humanized 4C11 monoclonal antibody (NET1-H-mAb) HUM03. raw

Pepscan data obtained by screening humanized 4C11 mAb against the over-lapping peptide library of 590 linear peptides (x-axis). The signal strength of the interaction is visualized on the y-axis.

Publications