NeuroMab™ Anti-Siglec-7 BBB Shuttle Antibody, Clone 10B5

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human; Cynomolgus Monkey; Mouse

Clonality

Monoclonal

Host Species

Mouse; Humanized

Clone Number

10B5

Applications

FC; ELISA; Block; In Vitro; In Vivo; Antagonist

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

Siglec 7

Official Name

SIGLEC7

Full Name

Sialic Acid Binding Ig Like Lectin 7

Alternative Names

Sialic Acid Binding Ig Like Lectin 7; QA79 Membrane Protein; SIGLEC-7; D-Siglec; AIRM-1; CDw328; AIRM1; P75; Sialic Acid Binding Ig-Like Lectin 19; Pseudogene; Adhesion Inhibitory Receptor Molecule 1; Siglec-7; Sialic Acid Binding Ig-Like Lectin; Pseudogene 2;

Product Pictures

FCM

Fig.1 shows FACS analysis of binding of Siglec-7 antibody to human primary dendritic cells compared to isotype control.

FCM

Fig.2 anti-Siglec-7 antibodies 4E3 and 10B5 were able to down-regulate cell surface expression of Siglec-7 on primary human dendritic cells.

Inhib

Fig.3 anti-Siglec-7 antibodies 10B5 and 4E3 inhibit PD-L1 and PD-L2 (PD-1 ligand), B7-H3 (strong T cell checkpoint inhibition on the MDSC surface. Substance), CD200R (inhibitory signaling receptor), and CD163 and CD206 (M2-type markers).

Endpoints after 30h were normalized to isotype control (grey bar) and % aggregated TDP-43 was calculated for ACI-7069- 633B12-AM (dotted grey bar). Mean values ± SD are shown for three independent experiments and statistical differences between isotype control and ACI-7069-633B12-AM (IgG2a variant) were analyzed by Welch's t-tests (***p < 0.001).

Publications