NeuroMab™ Anti-Tau; pTau Antibody, Clone 1H5
Functional antibody against Human, Mouse Tau, pTau
- Host Species:
- Mouse
- Species Reactivity:
- Human; Mouse
- Applications:
- WB; IHC; ICC; In Vitro; In Vivo
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Species Reactivity
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Host Species
Clone Number
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Preservatives
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Purity
Endotoxin Level
Low Endotoxin < 1 EU/mg
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Research Use Only
Figure 1 shows the characterization of the specificity of the tau antibody by immunoblot analysis of total brain lysates from nTg, tau KO, and PS19 tau Tg mice using actin as a control.
Brains from tau KO, nTg, and PS19 tau Tg mice were harvested and lysed in 2% SDS/50 mM Tris pH 7.5 as described in "Material and Methods". Equal amounts of proteins (40 μg) from each sample was resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with each antibody indicated above. Arrows depict human tau expressed in PS19 mice. The mobilities of molecular mass markers are shown on the left.
Figure 2 shows the specificity of tai antibodies raised against the tau peptide p193-211, as determined by immunoblotting with recombinant tau protein phosphorylated in vitro by p42 MAPK or GSK3β.
Figure 3 shows the biochemical characterization of sarcosyl-insoluble tau detected by a tau antibody in human brain lysates from AD patients.
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