Product

Delta Opioid Receptor Internalization Assay Cell Line

[CAT#: NCL2110P275]

Applications:: GPCR Internalization

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

Cell line information: DOR-tGFP_U2OS
Official Full Name: Delta Opioid receptor (DOR)
DNA Accesion Number: Gene Bank NM_000911
Host Cell: U2OS
Resistance: G418
Storage: Liquid Nitrogen

Features

The DOR-tGFP U2OS cell line contains 3 million U2OS cells, which stably express the human Delta opioid receptor (DOR) labeled with tGFP protein at the N-terminal. The Creative Biolabs DOR redistribution cell line is designed to detect potential agonists/antagonists against DOR and regulate its activation and subsequent redistribution processes within the cell. This cell line will allow image analysis of compound-induced stimuli. In high content analysis (HCA), the use of Deltorphin II as a DOR agonist has validated this highly reproducible assay.

Applications

GPCR Internalization

Research Areas

GPCR

Assay

Delta Opioid Receptor Internalization Assay

Assay Description

The Delta opioid receptor internalization analysis from Creative Biolabs allows the detection of compounds or their ability to modulate Delta opioid receptor (OPRD1) activation and the following redistribution processes within the cell. When the ligand binds to OPRD1, it activates G Protein, which is internalized in large, high-strength vesicles.

The assay has measured fluorescence OPRD1 redistribution by increasing the fluorescence around the nucleus using image analysis algorithms. The high reproducibility of this assay allows monitoring of the delta opioid receptor redistribution process in high-throughput screening.

Assay Characteristics

Readout: Fluorescent Receptor Internalization

Agonist: Deltorphin I

EC50 Agonist: 3.60 x 10⁶-10 M for receptor internalization

Assay type: Cell-based

Detection Method: Fluorescent Redistribution

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.