NeuroMab™ Anti-TLR1 BBB Shuttle Antibody, Clone GD2.F4
- Host Species:
- Mouse
- Species Reactivity:
- Human; Cynomolgus Monkey; Mouse
- Applications:
- ELISA; Block; Inhib; In Vitro; In Vivo
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryDescription
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
Species Reactivity
Clonality
Host Species
Clone Number
Applications
Storage
Research Use Only
Target
Official Name
Full Name
Alternative Names
Fig.1 is a bar graph depicting the results of enzyme linked immunosorbant assays (ELISAs) of the level of IL-6 expression in the presence or absence of TLR1 mAb (GD2.F4) or TLR2 mAb (11G7) in human PMBCs in the presence or absence of araLAM or PaM3CSK4.
Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.
Fig.2 is a graph depicting the results of experiments in which the level of IL-8 expression (pg/ml) was assayed in the presence of varying concentrations of control antibody (OKT8), anti-CD14 (26ic), anti-TLR1 (GD2.F4), anti-TLR2 (TL2. 1), and both anti-TLR1 and anti-TLR2.
Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.
Fig.3 is a bar graph depicting the results of ELISAs assaying the level of IL-6 expression in human PMBCs in the presence or absence of TLR1 mAb (GD2.F4) or TLR2 mAb (11G7), and in the presence or absence of zymosan or pLPS.
Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.
Fig.4 is a graph depicting the results of experiments in which the level of IL-8 expression was assayed in the presence of anti-TLR1 (GD2.F4), anti-TLR2 (TL2.1), and control IgG or anti-TLR1, anti-TLR2, and anti-CD14 monoclonal antibodies. TLR1 and TLR2 antibodies were provided at 0.2 μg/ml. Varying concentrations of isotype control or anti-CD14 antibodies were used.
Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.
Publications (0)
