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Creative Biolabs
Product

Irradiated Mouse Embryonic Fibroblasts (iMEF)

[CAT#: NCC20-9PZ51]

Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells

Species:
Mouse
Cell Types:
Other Cells

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Certificate of Analysis Lookup
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Product Overview

Description

The irradiated mouse primary embryonic fibroblasts (iMEF) at Creative Biolabs were dissociated from CD1 mouse embryos on the 14th and 15th days, expanded, and then inactivated by gamma radiation. Each vial contains at least 2 million cells.

Cell Types

Other Cells

Application Notes

• Guaranteed through 10 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease

Research Areas

Neurotoxicity; Neurogenesis

Species

Mouse
Properties

Size

≥ 2 million cryopreserved, gamma irradiated cells per ampule

Form

Cryopreserved

Shipping

Dry Ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Quality Control

• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased
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For Research Use Only. Not For Clinical Use.
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