Product
Human Preadipocytes (HPAd) Pre-Screened , Subcutaneous, Adult
[CAT#: NCL-2103-P47]
Positive for lipid drops in cytoplasm & Oil Red O Staining, in Differentiation Med
- Species:
- Human
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
HPAd are prescreened for Adipogenesis Signaling: Primary cultures of adipocytes are prepared by inducing differentiation of human primary preadipocytes and are prescreened for the expression of the following major adipocyte markers:
PPARγ (Peroxisome proliferator-activated receptor gamma), an adipocyte-specific nuclear hormone receptor
C/EBPα (CCAAT/enhancer binding protein alpha), a transcription factor involved in creating and maintaining the adipocyte phenotype
IR (Insulin receptor), which plays a critical role in induction of adipocyte differentiation, as well as functional regulation
ACCα (Acetyl-CoA carboxylase alpha), a key enzyme for de novo fatty acid synthesis and lipogenic capability of adipocytes
GSK-3β (Glycogen synthase kinase-3-beta), a ubiquitous kinase implicated in regulation of adipocyte gene expression, signaling, adipogenesis, insulin action and glycogen synthesis
Adiponectin, a bioactive adipokine specifically secreted by differentiated adipocyte and involved in metabolic regulation
PPARγ (Peroxisome proliferator-activated receptor gamma), an adipocyte-specific nuclear hormone receptor
C/EBPα (CCAAT/enhancer binding protein alpha), a transcription factor involved in creating and maintaining the adipocyte phenotype
IR (Insulin receptor), which plays a critical role in induction of adipocyte differentiation, as well as functional regulation
ACCα (Acetyl-CoA carboxylase alpha), a key enzyme for de novo fatty acid synthesis and lipogenic capability of adipocytes
GSK-3β (Glycogen synthase kinase-3-beta), a ubiquitous kinase implicated in regulation of adipocyte gene expression, signaling, adipogenesis, insulin action and glycogen synthesis
Adiponectin, a bioactive adipokine specifically secreted by differentiated adipocyte and involved in metabolic regulation
Cell Types
Other Cells
Species
Human
Properties
Tissue Source
Normal healthy human adipose tissue
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
