NeuroMab™ Anti-EPHA2 BBB Shuttle Antibody,Clone NR2685P
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- WB; In Vitro; In Vivo; Inhib; Agonist
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Figure 1 EphA2 antibody inhibits malignant tumor cell growth in vitro. Purified EphA2 antibody was incubated with malignant and benign tumor15 cells in soft agar at 37°C for 7 days.
A549 malignant lung cancer cells were incubated with 10 μg/ml or 2.5 μg/ml of EA2 or EA5 monoclonal antibodies or control (PBS). All antibody amounts used inhibited cell growth in soft agar.
Figure 2 EphA2 antibody EA2 inhibits tumor cell growth in vivo.
MDA-MB-231 breast cancer cells were implanted subcutaneously in athymic mice. After tumors grew to a mean volume of 30 to 100 mm3, mice were administered 6 mg/kg of the indicated antibody or negative control intraperitoneally twice weekly for 3 weeks. Total tumor volume was determined after sacrifice. Negative control is black and EA2 is white.
Figure 3 EphA2 agonistic antibody EA2 reduces malignant growth.
1x105 control or MCF-7EPhA cells were suspended in soft agar in the presence or absence of tamoxifen (TAM, 1 pM) and EphA2 agonistic antibody (EA2, 10 μg/ml). Note that EA2 increased the sensitivity of MCF-7EIhA2 cells to tamoxifen.
Figure 4 The EphA2 EA2 epitope is distinct from the ligand binding site.
EphA2-F was incubated with and bound to 5 immobilized Ephrin Al-Fe. Labeled EA2 was then incubated with the EphA2-ephrin A1 complex. Unlabeled competitors were incubated with the indicated amounts of EphA2-Ephrin A1-EA2 complexes. Competitors are Ephrin Al-Fc (black) or EA2 (white).
Figure 5 Estrogen receptor expressed but functionally altered in 30 MCF-73EPA cells.
E2 indicates estrogen treatment; TAM indicates tamoxifen treatment; % conversion indicates the amount of substrate converted from non-acetylated substrate (non-AC) to acetylated substrate (AC) by 5 CAT enzyme.
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