Product
Human EPHA2 Knockout HCT116 Cell Lysate
[CAT#: NCL2008ZP90]
Human EPHA2 knockout cell lysate
- Species:
- Human
- Cell Types:
- HCT116 Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a HCT116 cell transfected lysate in which Human EPHA2 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HCT116 Cell
Cell Location
Epithelial
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and Insertion of the selection cassette in exon5.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Research Areas
Neurotransmission
Pathways
Intracellular Signaling
Marker
Tyrosine Kinase Receptors
Species
Human
Properties
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target Details
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
