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Creative Biolabs

Human EPHA2 Knockout HCT116 Cell Lysate

[CAT#: NCL2008ZP90]

Human EPHA2 knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HCT116 cell transfected lysate in which Human EPHA2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and Insertion of the selection cassette in exon5.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Intracellular Signaling

Marker

Tyrosine Kinase Receptors

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

EPHA2

Official Name

EPHA2

Full Name

EPH receptor A2 (ephrin type-A receptor 2)

Alternative Names

EPHA2; Epha2; AW545284; Eck; Myk2; Sek-2; Sek2; ARCC2; CTPA; CTPP1; CTRCT6; EPH receptor A2; ECK

Gene ID

1969(Human)

Uniprot ID

P29317(Human)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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