NeuroMab™ Anti-IGF1R Antibody, Clone 12B1

Product Overview

Description

12B1 antibody recognizes an epitope on IGF-1R but fails to specifically bind the insulin receptor (IR).

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

12B1

Applications

IHC; WB

Application Notes

WB: 0.2-2 µg/ml, IHC: 5-20 µg/ml, IF/ICC: 5-20 µg/ml. Optimal dilutions/concentrations should be determined by the end user.

Conjugation

Unconjugated

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

IGF1R

Official Name

IGF1R

Full Name

insulin-like growth factor 1 (IGF-1) receptor

Alternative Names

IGF1R; CD221; IGFIR; IGFR; JTK13; insulin like growth factor 1 receptor; Insulin-like growth factor 1;IGF-1R

Gene ID

3480(Human); 16001(Mouse); 25718(Rat)

Uniprot ID

P08069(Human); Q60751(Mouse); P24062(Rat)

Product Pictures

FCM

Figure 1 shows the histogram of the FACS analysis detailing the binding of various IGF-1R-specific antibodies to IGF-1R.

Figure 2 depicts Western blot analysis of NIH 3T3 IGF-1R+ membrane extracts and recombinant extracellular domain of IGF-1R and IR probed with mAb 12B1 after SDS-PAGE electrophoresis under non-reducing and reducing conditions.

Inhib

Figure 3 shows the 125IIGF-1 binding inhibition experiment. Plot total specific IIGF-1 binding (%) as a function of ligand concentration on a semi-log graph. Specific binding values are the mean of experiments performed in triplicate.

IHC

Figure 4 shows the results of immunohistochemical studies (IHC) of various cell lines using a control antibody (IgG) and 12B1. The data show that 12B1 differentially stains membranes of various cell lines.

IHC

Figure 5 Upper panel: Goat polyclonal IGF-1R IHC (@ 1.0 μg/ml) in colon cancer. 20x (left) and 40x (right - higher magnification image of inset) objectives. Tumor cells have variable membrane staining. Bottom: 12B1 monoclonal IGF-1R IHC (@ 0.75 μg/ml) in colon cancer.

IHC

Figure 6 Left panel: Goat Poly IGF-1R IHC (@ 1.0 μg/ml) in colon cancer #2. 40× objective lens.

Tumor cells have plasma membrane staining. Right: 12B1 monoclonal IGF-1R (@ 0.75 μg/ml) in colon cancer #2 in the same tumor area shown on the left. 40× objective lens. Plasma membrane and apical globular, Golgi-like staining were present in tumor cells.

IHC

Figure 7 Top left: Goat polyclonal IGF-1R IHC (@ 2.0 μg/ml) in normal skin #3. 40× objective lens. Staining of the epithelial cell membrane on the outer side of the hair follicle (light/thin arrow).

Publications