NeuroMab™ Anti-IL1RAP BBB Shuttle Antibody,Clone NR2461P

Figure 1 Binding of exemplary antibodies to human IL-1RAP in an indirect ELISA. The exemplary antibody CAN04 of the present invention is known to have the highest affinity for human IL-IRAP.

Figure 2 Binding of an exemplary antibody of the invention (CAN04) to human CML and AML cells.

Figure 3 Ability of exemplary antibody CAN04 to block IL-1α signaling.

Figure 4 CAN04 in IF.

Combined with CAN04-AF488 conjugated antibody (green) and incubated for 2 hours on ice, or 2 hours at 37 ° C, or with CAN04-AF488 conjugated antibody (green) Confocal image (one optical section is about 0.9 [mu] m thick) representing LAMA cells incubated for 16 hours. Clearly visible antibodies bound to the cell membrane of the majority of cells are observed after 2 hours of incubation on ice (" Ice 2h "). After incubation with CAN04-AF488 conjugated antibody (green) for 2 hours at 37 ° C, in addition to membrane binding, the antibody began to enter the cell (internalization) and localized in the cell substrate.

Figure 5 The effect of low fucose and Fc mutations on ADCC activity was determined using SKMEL5 cells.