NeuroMab™ Anti-S100 Beta Antibody,Clone NR357P

Product Overview

Description

The anti-S1OOB monoclonal antibody 1C8 exhibits binding specificity for the S100B protein both in vitro and in vivo.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Clone Number

NR357P

Applications

Neut; Block; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease; Amyotrophic Lateral Sclerosis; Ischemic Stroke; Epilepsy

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

S100Beta

Official Name

S100B

Full Name

S100 calcium-binding protein B

Alternative Names

S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

Gene ID

6285(Human); 20203(Mouse); 25742(Rat)

Uniprot ID

P04271(Human); P50114(Mouse); P04631(Rat)

Product Pictures

ELISA

Figure 1 Dose-response curves of engineered antibodies.

Indirect ELISA was used to detect muBL356(lC8) in PBS and serum. Data are expressed as mean percent binding ± SEM (n=3). The shift in the dose-response curve indicates that quantitative data can be obtained if the control and experimental samples contain equivalent serum concentrations.

FuncS

Figure 2 In vivo pharmacodynamics/target binding of engineered antibodies.

Representative photomicrographs of brain sections from 6-month-old PSAPP mice treated with a single dose of engineered antibody (BL356) or placebo/control for 7 days. Sections were subjected to proximity junction assay using S100B + TLR4 primary antibody (brown; solid arrow) and counterstained with hematoxylin to visualize nuclei (blue; dashed arrow). Bar = 50 μm.

Publications