NeuroMab™ Anti-Tau BBB Shuttle Antibody, Clone H150

Figure 1 shows the specificity of tau antibodies raised against the tau peptide p391-409, as determined by immunoblotting of recombinant tau protein phosphorylated in vitro by p42 MAPK or GSK3β.

Figure 2 shows the characterization of the specificity of the tau antibody by immunoblot analysis using whole brain lysates from nTg, tau KO, and PS19 tau Tg mice using actin as a control.

Brains from tau KO, nTg, and PS19 tau Tg mice were harvested and lysed in 2% SDS/50 mM Tris pH 7.5 as described in "Material and Methods". Equal amounts of proteins (40 μg) from each sample was resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with each antibody indicated above. Arrows depict human tau expressed in PS19 mice. The mobilities of molecular mass markers are shown on the left.

Figure 3 shows the specificity of tai antibodies raised against the tau peptide p193-211, as determined by immunoblotting with recombinant tau protein phosphorylated in vitro by p42 MAPK or GSK3β.