Tau Phosphorylation Assay Cell Line

[CAT#: NCL2110P254]

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: Tau0N4R-TM-tGFP U2OS Stable Cell line (Alzheimer's disease in vitro model)
Pathway: Tau De/Phosphorylation - Microtubule De/Binding
Assessment: Tau & Microtubule Bundle Formation Assessment
HCS Application: Fluorescent Bundle Quantification
Material provided: Stable Cell Line (2 vial of cells); Division-Arrested Cells (2 million cells)

Features

Multiple Tau gene mutations are the causative factors of inherited FTDP-17 disease. These mutations have a similar effect to the hyperphosphorylation of Tau in AD and lead to the formation of NFT. U2OS stably expressing triple mutant (TM) human ON4R Tau-tGFP can be used to evaluate endogenous Tau kinase and phosphatase in living cells. The combination of Tau and MT and continuous bundle formation depend on phosphorylation: hyperphosphorylation of Tau causes it to dissociate from the microtubules and aggregate into tangles of paired spiral filaments (PHF). In contrast, kinase inhibitors promote Tau to bind to microtubules and form microtubule bundles. Automatic image analysis is used to detect and quantify this beam increase through fluorescence. It is suitable for monitoring the disease process in the body in the bioimaging system.

Neurological Disease Models

AD-related Cells

Cell Types

Other Cells

Relevant Diseases

Alzheimer's Disease; CNS

Assay

tau Phosphorylation Assay

Assay Description

Creative Biolabs' tau phosphorylation assay allows identification of tau kinase and phosphatase inhibitors and modulators that affect the behavior or location of tau protein. In this tau phosphorylation assay, we use a U2OS cell line stably expressing triple mutant (TM) human 0N4R Tau-tGFP. The binding of tau to microtubules and continuous bundle formation depend on phosphorylation. Hyperphosphorylation of tau causes it to dissociate from the microtubules and aggregate into tangles of paired helical filaments (PHF). In contrast, kinase inhibitors promote the binding of tau to microtubules and the formation of microtubule bundles.

Assay Characteristics

Readout: tau & microtubule Bundle Formation

Reference Cpd: LiCl

IC50: 11.75 mM

Type of assay: Cell based / functional

Detection Method: Image Analysis

Species

Human

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.