Rat microtubule-associated protein tau (Mapt) (NM_017212) ORF clone, Untagged

Description

This is a rat MAPT untagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

MAPT

Species

Rat

Insert Size (bp)

1125 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCTGAACCCCGCCAGGAGTTTGACACAATGGAAGACCAGGCCGGAGATTACACTATGCTCCAAGACCAAGAAGGAGACATGGACCATGGCTTAAAAGCTGAAGAAGCAGGCATCGGAGACACCCCGAACATGGAGGACCAAGCTGCTGGGCATGTGACTCAAGCTCGAGTGGCCGGCGTAAGCAAAGACAGGACAGGAAATGACGAGAAGAAAGCCAAGGGCGCCGATGGCAAAACGGGGGCGAAGATCGCCACACCTCGGGGAGCAGCCACTCCGGGCCAGAAAGGCACATCCAATGCCACCAGGATCCCAGCCAAGACCACACCCAGCCCAAAGACTCCTCCAGGATCAGGTGAACCACCAAAATCCGGAGAACGAAGCGGCTACAGCAGCCCCGGCTCGCCCGGAACCCCTGGCAGTCGCTCCCGTACCCCATCCCTACCAACGCCGCCCACCCGAGAGCCCAAAAAGGTGGCAGTGGTTCGCACTCCCCCTAAGTCACCGTCTGCCAGTAAGAGCCGCCTACAGACTGCCCCTGTGCCCATGCCAGACCTAAAGAACGTCAGGTCCAAGATTGGCTCCACTGAGAACCTGAAGCACCAGCCGGGAGGCGGCAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAACGTCCAGTCCAAGTGTGGCTCAAAGGACAATATCAAACACGTCCCGGGCGGAGGCAGTGTGCAAATAGTCTACAAGCCAGTGGACCTGAGCAAGGTGACCTCCAAGTGTGGTTCCTTAGGGAACATCCATCACAAGCCAGGAGGTGGCCAGGTAGAAGTAAAATCAGAGAAGCTGGACTTCAAGGATAGAGTCCAGTCGAAGATTGGCTCCTTGGATAACATCACCCATGTCCCTGGAGGAGGGAATAAGAAGATTGAAACCCACAAGCTGACCTTCAGGGAGAATGCCAAAGCCAAGACAGACCATGGAGCAGAAATCGTGTACAAGTCACCTGTGGTGTCTGGGGACACATCTCCACGGCACCTCAGCAACGTCTCCTCCACGGGCAGCATCGACATGGTGGACTCTCCACAGCTTGCCACGTTAGCCGATGAAGTGTCCGCCTCTTTGGCCAAGCAGGGTTTGTGAACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Growth and Development; Neuroregeneration

Relevant Diseases

Neurodegenerative Disease; Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

Tau

Official Name

MAPT

Full Name

Microtubule-associated protein

Alternative Names

MAPT; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; microtubule associated protein tau; Tau proteins; tau-40

Gene ID

4137(Human); 17762(Mouse); 29477(Rat)

Uniprot ID

P10636(Human); P10637(Mouse); P19332(Rat)