NeuroMab™ Anti-TDP43 BBB Shuttle Antibody, Clone TDP-O-10

Figure 1 depicts that TDP-0 mAb exhibits higher specificity for TDP-43 oligomers.

Various concentrations of TDP-0 mAbs (1 -2 x 10"^g/mL) respectively produced by TDP-O-3, -5, -8, -9, and -10 hybridoma cells were used in ELISA assay to detect the SDS denatured or non-denatured TDP-43.

Figure 2 depicts that TDP-0 mAb exhibits higher specificity for purified TDP-43 oligomers.

Conditional medium of TDP-O-3, -5, -8, -9, and -10 hybridoma cell lines were used to detect the TDP-43 oligomers and monomers by ELISA assay.

Figure 3 depicts TDP-0 monoclonal antibody rescue of TDP-43 oligomer-induced cytotoxicity.

MTT assay was performed to examine cell viability of BE(2)-C cells. Data are presented as mean ± standard deviation. Statistical analysis was performed by one-way ANOVAs, *p< 0.05, **p< 0.01 , ***p< 0.001 . The result showed the toxicity induced by TDP-43 oligomers was significantly rescued by the treatment of TDP-0 antibody.

Figure 4 depicts immunogold labeling of TDP-43 oligomers immunoprecipitated from diseased hippocampus.

zoom-in images of TDP-43 oligomers (scale bar, 50 nm).

Figure 5 depicts the presence of TDP-43 oligomers in FTLD-TDP patients.

A total of three FTLD-TDP cases, three neurologically and pathologically normal age-matched controls, and three Alzheimer's disease cases without TDP-43 inclusions (as "neurodegenerative disease controls") were examined .

Figure 6 depicts that TDP-43 oligomers are present and increase with age in a transgenic mouse model of FTLD-TDP.

Immunofluorescent staining of human TDP-43 and TDP-43 oligomers in brain sections of wild-type and 6- and 12-month-old TDP-43 Tg+/+ transgenic mice. Cells with anti-TDP oligomer staining, TDP-43 staining, and DAPI staining are shown (scale bar, 100 μm). Closed regions are presented at higher magnification in the last column (scale bar, 25 μm).

Figure 7 depicts TDP-0 antibody specific recognition of TDP-43 oligomers.

Figure 5D: 1 ml SEC fractions were collected and dot blotted with TDP-0 (upper blot) and N-26o antibodies (lower blot).