Product

TDP-43 Stress Granules Assay Cell Line

[CAT#: NCL2110P249]

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Product Overview

Description

Cell line information: TDP43-tGFP/ U2O2 Cell line (ALS in vitro model)
Official Full Name: TAR DNA-binding protein 43
DNA Accesion Number: GenBank NM_000176
Host Cell: U2OS
Format: Cryopreserved vials

Features

Each vial contains U2OS cells stably expressing human TAR DNA binding protein 43 (TDP-43) labeled with tGFP. Creative Biolabs' TDP43 translocation detection cell line is designed to detect the ability of compounds or analytical stimuli to modulate TDP-43 translocation, follow the cytoplasmic translocation process and quantify intracellular fluorescence accumulation. This highly reproducible assay allows monitoring of TAR DNA-binding protein 43 translocation in high-content analysis and fluorescence microscopy applications.

Relevant Diseases

Amyotrophic Lateral Sclerosis; CNS

Assay

TDP-43 Stress Granules Assay

Assay Description

Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative diseases of the motor neuron system. TDP-43 pathology appears to be the main pathological type of sporadic ALS types. Our TDP-43 stress granule assay allows pathological quantification TDP43 spheres enter the nucleus and cytoplasm. This model also allows monitoring the distribution of TDP-43 protein in living cells, studying the protein localization pattern in space and time, and quantifying the intracellular fluorescence aggregation.

For this assay, we use a stable cell line expressing fluorescent TDP-43. During the assay, the cells were incubated with the test compound and sodium arsenite to induce TDP-43 aggregation. Finally, we used BD Pathway HCS Reader and Attovision Compartimentalization software to quantify the TDP43-tGFP nucleosphere.

Assay Characteristics

Readout: TDP-43 Stress Granules quantification

Agonist: Sodium Arsenite

EC50 Agonist: 7.3 x 10⁶-5 M

Positive Compound 1: Arimoclomol

Positive Compound 2: Riluzole

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.