Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit

Name: Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit
SKU: NRZP-0722-ZP238
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP238]

For the quantitative determination of human TAR DNA-binding protein 43 (TARDBP/TDP43) concentrations in serum, plasma, cell culture supernates, cerebrospinal fluid (CSF).

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human TARDBP.

Species Reactivity

Human

Relevant Diseases

Amyotrophic Lateral Sclerosis

Marker

TAR DNA binding protein

Properites

Sensitivity

In most cases, the sensitivity is below 0.078 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cell culture supernates, cerebrospinal fluid (CSF)

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.312 ng/ml-20 ng/ml

Max Emission

450 nm

Target Details

Target

TDP43

Official Name

TARDBP

Full Name

TAR DNA binding protein

Alternative Names

TAR DNA-binding protein 43; TDP-43; ALS10

Gene ID

23435(Human)

Uniprot ID

Q13148(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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