Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA Kit
[CAT#: NRZP-0722-ZP238]
For the quantitative determination of human TAR DNA-binding protein 43 (TARDBP/TDP43) concentrations in serum, plasma, cell culture supernates, cerebrospinal fluid (CSF).
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human TARDBP.
Species Reactivity
Human
Relevant Diseases
Amyotrophic Lateral Sclerosis
Marker
TAR DNA binding protein
Properites
Sensitivity
In most cases, the sensitivity is below 0.078 ng/ml.
Detection Method
Sandwich
Sample Type
serum, plasma, cell culture supernates, cerebrospinal fluid (CSF)
Assay Type
quantitative
Assay Duration
1-5h
Number Of Samples
50-100ul
Detection Range
0.312 ng/ml-20 ng/ml
Max Emission
450 nm
Target Details
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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Publications (0)
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