NeuroMab™ Anti-IGF1R Antibody, Clone EM164

Product Overview

Description

The antibody specifically binds to, and inhibit, insulin-like growth factor-I receptor, antagonize the effects of IGF-I, IGF-II and serum on the growth and survival of tumor cells, and which are substantially devoid of agonist activity. The antibody can be used in the treatment of tumors that express elevated levels of IGF-I receptor, such as breast cancer, colon cancer, lung cancer, ovarian carcinoma, synovial sarcoma, prostate cancer.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Clone Number

EM164

Applications

ELISA; FC; Inhib; In Vivo; In Vitro

Application Notes

ELISA: 1/2000 - 1/5000, WB: 1/500 - 1/2000, IHC: 1/100 - 1/300. Optimal dilutions/concentrations should be determined by the end user.

Conjugation

Unconjugated

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

IGF1R

Official Name

IGF1R

Full Name

insulin-like growth factor 1 (IGF-1) receptor

Alternative Names

IGF1R; CD221; IGFIR; IGFR; JTK13; insulin like growth factor 1 receptor; Insulin-like growth factor 1;IGF-1R

Gene ID

3480(Human); 16001(Mouse); 25718(Rat)

Uniprot ID

P08069(Human); Q60751(Mouse); P24062(Rat)

Product Pictures

FCM

Figure 1 shows fluorescence-activated cell sorting (FACS) analysis of the specific binding of purified EM164 antibody to cells overexpressing the human Y1251F IGF-I receptor or the human insulin receptor.

Inhib

Figure 2 shows the inhibitory effect of EM164 antibody on the binding of biotinylated IGF-I to human breast cancer MCF-7 cells.

FuncS

Figure 3 shows the effect of EM164 antibody on the growth and survival of MCF-7 cells under different growth conditions, evaluated by MTT assay.

FuncS

Figure 4 shows the effect of treatment with EM164 antibody, paclitaxel or a combination of EM164 antibody and paclitaxel on the growth of mouse Calu-6 lung cancer xenografts.