Mouse Anti-GAPDH Monoclonal Antibody (CBP6625)

Product Overview

Description

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing a step in the glycolytic pathway, which is the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate to glyceraldehyde diphosphate.. Because GAPDH protein is expressed in large quantities and is always required for important "housekeeping" functions, the level of GAPDH mRNA is often measured and used as a standard for other mRNA expression studies. Similarly, a specific antibody against GAPDH is used to measure the expression of GAPDH, which can be used as a protein standard in Western blot experiments to compare the level of this protein with other proteins in cells or tissues. In addition to its role in glycolysis, GAPDH may also have other roles, such as transcription activation (1), and bind to a variety of other proteins, including amyloid precursor protein, huntingtin's polyglutamine bundle ( 2,3). This protein may also play a role in the regulation of apoptosis. Interestingly, when a cell is apoptotic, it migrates from the cytoplasm to the nucleus (4). The control of this is mediated by the NO-mediated S-nitrosylation of GAPDH in the cytoplasm, which then binds and stabilizes the Siah1 E3 ubiquitin ligase, which enters the nucleus together with the nitrosylated GAPDH and initiates specific The protein degradation event caused the nuclear cell to activate the death pathway (5). Recently, GAPDH has been shown to be a substrate of 5'-AMP-dependent protein kinases, causing cellular GAPDH to redistribute to the plasma membrane and inhibit intracellular transport, which is a component of the cell starvation response (6).

Immunogen

Full-length protein purified from porcine blood cells

Species Reactivity

Human; Rat; Mouse; Cow; Porcine; Horse; Monkey; Dog; Chicken

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgM

Clone Number

CBP6625

Applications

WB; IF; IHC

Application Notes

WB: 1:1000
IF/IHC: 1:100

Product Properties

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Store at 4°C for short term, for longer term at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

GAPDH

Official Name

GAPDH

Full Name

Glyceraldehyde-3-Phosphate Dehydrogenase

Alternative Names

Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase; 38 Kd Component; Aging-Associated Gene 9 Protein;