Mouse Anti-GAPDH Monoclonal Antibody (CBP1948)

Product Overview

Description

Mouse anti-human GAPDH antibody combined with inorganic phosphate and nicotinamide adenine dinucleotide (NAD) can recognize the 335 amino acid multifunctional protein glycerol triphosphate dehydrogenase (GAPDH). Its main function is to catalyze 3- Reversible oxidative phosphorylation of glyceraldehyde phosphate.). This reaction is an important energy production step in carbohydrate metabolism.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are related to oxidative stress, thereby mediating cell death. Further reports indicate that GAPDH binds to several proteins responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al., 1996).
The mouse anti-human GAPDH antibody recognizes human GAPDH as a band of ~36 kDa by Western blotting in a variety of human cell line lysates under reducing conditions.

Immunogen

Human GAPDH in myocardium

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1948

Applications

Application Notes

WB (1/1000)

Relevant Diseases

Huntington's Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS; 0.09% Sodium Azide

Preservatives

Yes

Concentration

0.1 mg/ml

Purification

Affinity chromatography on Protein A from tissue culture supernatant.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

GAPDH

Official Name

GAPDH

Full Name

Glyceraldehyde-3-Phosphate Dehydrogenase

Alternative Names

Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase; 38 Kd Component; Aging-Associated Gene 9 Protein;

Gene ID

2597(Human); 14433(Mouse); 24383(Rat)

Uniprot ID

P04406(Human); P16858(Mouse); P04797(Rat)

References

1. Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
2. Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
4. Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
5. Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.

Publications