Product
Mouse Anti-GAPDH Monoclonal Antibody (CBP1948)
[CAT#: NAB2007FY411]
Mouse Monoclonal [W0037] to GAPDH
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- WB
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
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Description
Mouse anti-human GAPDH antibody combined with inorganic phosphate and nicotinamide adenine dinucleotide (NAD) can recognize the 335 amino acid multifunctional protein glycerol triphosphate dehydrogenase (GAPDH). Its main function is to catalyze 3- Reversible oxidative phosphorylation of glyceraldehyde phosphate.). This reaction is an important energy production step in carbohydrate metabolism.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are related to oxidative stress, thereby mediating cell death. Further reports indicate that GAPDH binds to several proteins responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al., 1996).
The mouse anti-human GAPDH antibody recognizes human GAPDH as a band of ~36 kDa by Western blotting in a variety of human cell line lysates under reducing conditions.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are related to oxidative stress, thereby mediating cell death. Further reports indicate that GAPDH binds to several proteins responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al., 1996).
The mouse anti-human GAPDH antibody recognizes human GAPDH as a band of ~36 kDa by Western blotting in a variety of human cell line lysates under reducing conditions.
Immunogen
Human GAPDH in myocardium
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG1
Clone Number
CBP1948
Applications
WB
Application Notes
WB (1/1000)
Relevant Diseases
Huntington's Disease
Research Areas
Neuroregeneration
Conjugation
Unconjugated
Product Properties
Form
Liquid
Formulation
PBS; 0.09% Sodium Azide
Preservatives
Yes
Concentration
0.1 mg/ml
Purification
Affinity chromatography on Protein A from tissue culture supernatant.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Target
GAPDH
Official Name
GAPDH
Full Name
Glyceraldehyde-3-Phosphate Dehydrogenase
Alternative Names
Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase; 38 Kd Component; Aging-Associated Gene 9 Protein;
References
1. Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
2. Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
4. Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
5. Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
2. Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
3. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
4. Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
5. Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
Publications
Publications (0)
Neural Genes
Neural Antibodies
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
