DRG Sensory Neuron Ion Channel Assay

Creative Biolabs is a leading provider of research and development services in the neuroscience industry. Our company is committed to advancing the understanding of human biology through cutting-edge technologies and innovative solutions. One of our key areas of focus is the study of sensory neurons, which play a critical role in the perception and transmission of sensory information throughout the body. In particular, we specialize in the development and application of Dorsal Root Ganglia (DRG) Sensory Neuron Ion Channel Assays, a powerful platform for studying the function of ion channels in peripheral sensory neurons.

Peripheral Sensory Neurons Isolation and Cultures

Peripheral sensory neurons are a diverse group of cells that are responsible for detecting and transmitting sensory information throughout the body. We isolated DRG tissues from the spinal cord of rodents and then processed them into single-cell suspensions for sensory neuron culture. The cultured sensory neurons be used directly for electrophysiology, signal transduction, neurotransmitter release, or calcium imaging.

Fig.1 Protocol for dissection and culture of murine DRG neurons. (Perner, 2021)

Voltage Clamp Recording

The neurons found in the dorsal root ganglion (DRG) exhibit a diverse range of voltage-gated ion channels, which are responsible for regulating the transmission of sensory stimuli from peripheral regions to the spinal cord through the so-called 'pain gatekeeper' synapse in the dorsal horn. Therefore, the gold standard for electrophysiological recordings of neuronal ion channels is voltage clamp using manual patch clamp techniques. Creative Biolabs is capable of recording neuronal ion channels from native rodent neurons and from rodent and human neuroblastoma cell lines and has experience in being able to isolate ion channels of interest, utilizing our extensive biophysics and pharmacological knowledge.

Assay Applications

This assay has several key advantages over other methods of studying ion channels, including:

• High sensitivity: The patch clamp technique allows the precise measurement of ion channel currents in single cells, providing a highly sensitive method for studying ion channel function.
• Pharmacological profiling: The assay can be used to study the pharmacological sensitivity of ion channels, allowing researchers to identify novel compounds that modulate ion channel activity.
• Functional analysis: The assay provides a functional readout of ion channel activity, allowing researchers to study the impact of ion channel dysfunction on sensory neuron function.

Our Advantages

At Creative Biolabs, we have extensive experience in the development and application of DRG Sensory Neuron Ion Channel Assays. Our team of experts includes highly skilled neuroscientists and electrophysiologists who have a deep understanding of the function of ion channels in peripheral sensory neurons.

We pride ourselves on our ability to provide customized solutions to our clients, tailoring our assays to meet their specific research needs. Our state-of-the-art facilities and equipment allow us to provide high-quality data with rapid turnaround times,

If you are looking for reliable and efficient DRG neuron characterization services, get in touch with us today to find out more about how we can support you in your endeavors!

Reference

1. Perner, Caroline, and Caroline L. Sokol. "Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release." STAR protocols 2.1 (2021): 100333.