iNeu™ Human Schwann Cell
[CAT#: NCL-2103-P63]
- Species:
- Human
- Cell Types:
- Schwann Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
iNeu™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, genetic-vector-free, and integration-free conditions. They are maintained as a monolayer culture under defined cell culture conditions and can be co-cultured with neurons. iNeu™ expression biomarkers: GAP43, MPZ, NCAM1, NGFR, OCT6, Sox2 and Sox10 (mRNA analysis). Using immunocytochemistry, iNeu™ expresses S100 beta and MBP.
Cell Types
Schwann Cell
Application Notes
Applications:
• Glial cell differentiation and neuron-glia co-culture studies
• Disease modeling (e.g., Charcot-Marie-Tooth disease, Guillain-Barré syndrome)
• Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)
• Glial cell differentiation and neuron-glia co-culture studies
• Disease modeling (e.g., Charcot-Marie-Tooth disease, Guillain-Barré syndrome)
• Phenotypic Screening (e.g., synaptic release assays, inflammatory cytokines ELISA assays)
Species
Human
Properties
Size
~0.6x10^6 cells per 1ml of freezing medium (vial)
Growth Pattern
adherent
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Sterility, Safety, (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative; Cell viability post-thawing (>90%)
Neural Cell Lines
FQA
Do you provide related culture media for the iSchwann cells?
Yes, we provide Schwann Growth Medium, Cat.# NCC-2103-P65.
Do you have the HLA information for these cells?
Should you require comprehensive HLA characterizations for any donor or patient line, we offer the option of a customized project to cater to your specific needs.
For Research Use Only. Not For Clinical Use.
