iNeu™ Human Motor Neurons

[CAT#: NCL-2103-P71]

Species:: Human

Cell Types:: Motor Neurons

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Product Overview

Description

iNeu™ cells are reprogrammed from human iPSC-derived multipotent progenitor cells, using integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary serum-free, virus-free, nucleic-acids-free, feeder-free, and integration -free technology conditions. Motor neurons have synapses that are transmitted through cholinergic transmitters. These synapses require extremely rapid inactivation of the transmitter acetylcholine (Ach) by using the enzyme acetylcholinesterase (AchT). AchT hydrolyzes acetylcholine into acetate and choline as an inactivation method. iNeu™ cells are plated as a monolayer when cultured, showing a polarized structure and expressing biomarkers, acetylcholinesterase (AchT), β-TUJ (neuron specific), Isl1 and HB9.

Cell Types

Motor Neurons

Application Notes

Applications:

Disease modeling (e.g., ALS/Lou Gerig's, Motor Neuron Disorders)
Toxicity testing
Phenotypic Screening (e.g., co-culture with muscle tubules)
In vitro assays using motor neurons
Developmental biomarker and co-culture (with myotube) studies

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.