Amyotrophic Lateral Sclerosis (ALS) In Vitro Assay Services

Creative Biolabs offers cutting-edge In Vitro Assay Services specifically tailored for ALS research, providing invaluable insights into the underlying mechanisms of the disease and potential therapeutic strategies.

Cell Models

Creative Biolabs offers a comprehensive range of In Vitro assay models tailored to address various aspects of ALS pathogenesis. Our model list includes:

Cell Models	Details
Motor Neuron-Like Cell Lines	NSC-34: This is a widely used motor neuron-like cell line derived from mouse motor neurons. It has been extensively used for studying ALS-related molecular mechanisms and testing potential drug candidates. SH-SY5Y: Although derived from human neuroblastoma, these cells can be differentiated into motor neuron-like cells and used to study aspects of ALS pathology.
Primary Motor Neuron Cultures	Embryonic Rat Spinal Cord Cultures: Primary cultures of embryonic rat spinal cord can be used to study motor neuron development, function, and degeneration in a controlled environment. Induced Pluripotent Stem Cell (iPSC)-Derived Motor Neurons: iPSCs derived from ALS patients can be differentiated into motor neurons, providing a human-specific model for studying disease mechanisms and testing potential treatments.
Astrocyte and Glial Cell Cultures	Primary Astrocyte Cultures: Astrocytes play a significant role in ALS pathogenesis through their interactions with motor neurons. Cultures of primary astrocytes derived from mouse or human tissues can be used to investigate their contribution to disease progression. Co-Culture Systems: Co-culturing motor neurons with astrocytes or other glial cells can help mimic the complex interactions between different cell types in the ALS microenvironment.
Organoids and 3D Cultures	Brain and Spinal Cord Organoids: These three-dimensional cellular structures derived from iPSCs can model the architecture and function of the human brain and spinal cord, providing a more physiologically relevant environment for ALS studies.

ALS In Vitro Assays at Creative Biolabs

Our repertoire of ALS In Vitro assays includes a range of well-established protocols, each targeting distinct disease mechanisms. These assays are meticulously designed to provide quantitative readouts that enable precise evaluation of therapeutic interventions. Some of our featured assays include:

Motor Neuron Health and Viability

Neurite length and branching: Measure the extension and complexity of motor neuron projections, reflecting neuronal development and function.
Cell viability assays (MTT, CellTiter-Glo): Quantify the overall health and survival of motor neurons.

Protein Aggregation and Mislocalization

Detection of protein aggregates: Quantify the presence of aggregated proteins, such as such as mutant SOD1, TDP-43, FUS and C9orf72.
Subcellular localization: Assess the proper subcellular distribution of key ALS-associated proteins.

Oxidative Stress and Mitochondrial Function

Measurement of ROS levels: Evaluate the extent of oxidative stress in motor neurons.
Mitochondrial membrane potential (Δψm): Assess mitochondrial health and function.

Neuronal Excitability and Electrophysiology

Measurement of glutamate-induced excitotoxicity and calcium influx in motor neurons, enabling the identification of potential neuroprotective agents.
Electrophysiological recordings: Study changes in membrane potential, ion channel activity, and neuronal excitability in motor neurons.

Creative Biolabs stands at the forefront of ALS research, offering cutting-edge In Vitro assay services that empower researchers to unravel the intricacies of ALS pathology, accelerate drug discovery, and ultimately bring us closer to effective treatments for this devastating disorder. Our commitment to scientific excellence and innovation drives us to deliver unparalleled support to advance the field of neurodegenerative disease research.

For inquiries and collaborations, please don't hesitate to contact us for more information.

For Research Use Only. Not For Clinical Use.

Related Section

Stress Granule Formation Assay

C9orf72 RNA Foci Formation Assay

C9orf72 Di-peptide Aggregation Assay

Nucleo-cytoplasmic Transport Assay

TDP-43, FUS, SOD1 Localization & Aggregation Assay

ALS High-Throughput Phenotypic Screening Assay

ALS Therapeutic Target Characterization

In Vitro Services