Immune Cell Profiling in Neurological Diseases

Immune cell profiling involves the systematic characterization and quantification of immune cells within the nervous system. These cells play a pivotal role in both the pathology and potential therapy of neurological diseases. To address these challenges, Creative Biolabs employs cutting-edge technologies and expertise to provide in-depth immune cell profiling services. With our state-of-the-art infrastructure and team of experienced scientists, we are committed to advancing our understanding of the immune responses in neurological diseases and accelerating the development of novel therapeutic strategies.

High-Throughput Single Cell Analysis Platform

Creative Biolabs offers a high-throughput single-cell analysis platform that allows for the simultaneous profiling of thousands of individual immune cells. This technology enables a deeper understanding of the heterogeneity and diversity of immune cell populations within the nervous system. By analyzing each cell individually, we can identify rare cell subpopulations and study their functional characteristics.

Our platform utilizes multiple single-cell isolation techniques including microfluidics, fluorescence-activated cell sorting (FACS), flow cytometry, pico-wells, and laser microdissection. Isolated cells are then transferred for scRNA-Seq, combined with advanced bioinformatics tools to provide a comprehensive picture of immune cell function and gene expression. This enables us to identify potential biomarkers and therapeutic targets for neurological diseases.

Fig. 1 Overview of single-cell RNA Sequencing of the CSF.¹

Tetramer-based T Cell Isolation Service

T cells are crucial components of the adaptive immune system, and their role in neurological diseases has garnered significant attention. Creative Biolabs offers a T cell isolation service using tetramer-based technology, which allows for the isolation and analysis of antigen-specific T cells. This approach is particularly valuable for studying the immune response in conditions where specific antigens are implicated, such as multiple sclerosis.

TCR Repertoire Identification Service

T cell receptors (TCRs) are essential in recognizing specific antigens, and their diversity is a fundamental aspect of the adaptive immune system. In the context of neurological diseases, identifying the TCR repertoire and clonality within the nervous system is vital. Creative Biolabs employs state-of-the-art TCR sequencing techniques to provide in-depth TCR identification services.

By analyzing the TCR repertoire in various neurological diseases, we can uncover patterns and potential therapeutic targets. Additionally, this information is valuable for the development of immunotherapies, including CAR-T cell therapies, which hold promise for treating neuroinflammatory conditions.

HLA Typing Service

The human leukocyte antigen (HLA) system plays a pivotal role in immune responses by presenting antigens to T cells. HLA typing, a cornerstone of immune profiling, elucidates the genetic variability underlying individual immune responses to neurological diseases. Creative Biolabs offers comprehensive HLA typing services, ensuring that the HLA profiles of individuals are accurately determined. This information is indispensable in predicting the risk of developing neurological diseases, understanding disease mechanisms, and designing personalized immunotherapies.

Samples Types (CSF, PBMC)

The choice of sample source is crucial in immune cell profiling for neurological diseases. Creative Biolabs offers services for the analysis of cerebrospinal fluid (CSF) and peripheral blood mononuclear cells (PBMC). CSF analysis allows for a direct examination of the immune cells within the central nervous system, while PBMC analysis provides insights into the systemic immune response.

We understand the complex interplay between the immune system and neurological diseases, and our commitment to advancing the field is unwavering. Together, we are shaping the future of diagnosis and treatment for these challenging conditions. Don't hesitate to contact us for more information.

References

1. Yekula, Anudeep, et al. "Single-cell RNA sequencing of cerebrospinal fluid as an advanced form of liquid biopsy for neurological disorders." Brain Sciences12.7 (2022): 812.