Rat Immortalized Retinal Muller Cell Line rMC-1

Species:: Rat

Cell Types:: Visual system-related Cells

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Product Overview

Cell Types

Visual system-related Cells

Application Notes

Harvest cells with 0.05% trypsin for 5 min and replate

Species

Rat

Properties

Size

>1x10^6 Cells

Form

Frozen

Growth Pattern

Flat

Culture Medium

DMEM (high glucose, (+) L-Glutamine, (-) Sodium pyruvate) with 10% FBS and 1X pen/strep, with additional L-glutamine (2mM).

Freeze Medium

DMEM with 10% DMSO and 10%FBS

Biosafety Level

BSL1

Tissue Source

Retina

Shipping

Dry ice

Storage

Liquid Nitrogen

Research Use Only

This product is intended for research use only.

Publications

Publications (0)

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Consistent morphology and growth rate

One of the best features of the rMC-1 cell line is its consistent morphology and growth rate. We have not experienced any issues with contamination or variation in cell behavior, which has been critical for our long-term studies.

Excellent

Highly reproducible results

In our experience, the rMC-1 cells provide highly reproducible results. Whether we're measuring cell viability, proliferation, or gene expression, the data is consistent and reliable. This cell line has significantly improved the reliability of our experiments.

Excellent

Robust and easy to maintain

The rMC-1 cells are incredibly robust and easy to maintain. They grow rapidly and do not require overly complex culture conditions. This has made our workflow much more efficient, especially when scaling up for larger experiments.

Excellent

Excellent model for retinal research

As a researcher focused on retinal diseases, I find the rMC-1 cell line to be an excellent model. The cells exhibit key characteristics of Müller glial cells, making them ideal for studying retinal physiology and pathology.

Excellent

Cost-effective and efficient

The rMC-1 cell line is not only effective but also cost-efficient. The cells require standard culture media and conditions, reducing the overall cost of experiments. This has been beneficial for our lab's budget constraints.

Excellent

Excellent support from supplier

The support from the supplier has been excellent. They provided detailed protocols and were available to answer any questions we had. This level of support has been invaluable in getting our experiments up and running smoothly.

Q&As

How do rMC-1 cells compare to primary Müller cells in experiments?

rMC-1 cells offer the advantage of immortality, allowing for consistent long-term studies compared to primary Müller cells, which have limited lifespan and variability. However, primary cells may better represent the in vivo state. The choice depends on the study's focus; rMC-1 cells are excellent for mechanistic studies, while primary cells are ideal for physiological relevance.

How should I store rMC-1 cells for long-term use?

For long-term storage, cryopreserve rMC-1 cells in liquid nitrogen. Prepare a freezing medium consisting of DMEM with 10% DMSO and 10%FBS. Freeze the cells gradually using a controlled-rate freezing container before transferring to liquid nitrogen. Ensure that the cells are stored at -196°C, and check the viability post-thaw to confirm the cells' integrity.

Do you have any troubleshooting tips for culturing rMC-1 cells?

If you encounter slow growth, check the medium's pH, serum quality, and CO2 levels in the incubator. If cells detach or appear unhealthy, avoid over-confluence and optimize trypsinization conditions. For contamination issues, confirm the sterility of reagents and practice strict aseptic techniques. Regular monitoring and maintaining good cell culture practices will ensure optimal cell health and experimental outcomes.