Product
Aβ Peptide Formation/Screening Assay Cell Line
[CAT#: NCL2110P333]
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
H4 glioma cells overexpressing human APPK595N/M596L (Swedish double mutation) can analyze the compound's effect on the human mutant APP profile.
Primary rat/mouse hippocampal neurons are phenotypically closer to adult neurons because they present an expanded dense neuronal process network.
Primary chicken telencephalon neurons secrete endogenous wild-type Aβ peptides, including Aβ38, Aβ40, and Aβ42. Human and chicken Aβ42 are identical in sequence. Therefore, this model is suitable for studying the effect on endogenous wild-type APP processing in primary neurons.
Primary rat/mouse hippocampal neurons are phenotypically closer to adult neurons because they present an expanded dense neuronal process network.
Primary chicken telencephalon neurons secrete endogenous wild-type Aβ peptides, including Aβ38, Aβ40, and Aβ42. Human and chicken Aβ42 are identical in sequence. Therefore, this model is suitable for studying the effect on endogenous wild-type APP processing in primary neurons.
Relevant Diseases
Alzheimer's Disease
Assay
Aβ Peptide Formation/Screening Assay
Assay Description
The production of Aβ species is a hallmark of Alzheimer's disease. Creative Biolabs provides several cell culture models to analyze the production of different Aβ and sAPP species through immunosorbent assays to identify pharmacological reagents that interfere with APP lysis.
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
