Lipophilic Tracer Tools
Carbocyanine dyes, such as DiI and its derivatives, can label cell membranes without significantly affecting cell viability and can be used for long-term research. Carbocyanine dyes are widely used as lipophilic tracers to label cells, organelles, liposomes, viruses and lipoproteins. These dyes can be used for a variety of applications for long-term cell tracking, including cell transplantation, migration, adhesion and fusion research.
Advantages
- Exogenous staining
- Crystal and paste formulations for tissue staining
- Will stain fixed tissue sections
- Forward and retrograde transportation
Applications
Lipophilic tracers can be used to track neuronal projections in fixed tissue sections. The dye uniformly marks neurons through the lateral diffusion in the plasma membrane and is supplemented by the active dye transport process in living tissues. Lipophilic tracers can also be used for tissue sections, neurons in vivo, fixed tissue sections and cultured cells. These lipophilic nerve tracers can be used for anterograde and retrograde transport research.
In order to increase the slow diffusion rate of lipophilic tracers from application to neuron ends, Creative Biolabs provides lipophilic probes with unsaturated alkyl chains to speed up the diffusion process.
| Readout | Labels cell membranes and does not appreciably affect cell viability. The dyes uniformly label neurons via lateral diffusion in the plasma membrane, supplemented by active dye transport processes in living tissues. Crystal and paste preparations provide convenient loading optons for tissue sections. |
| Tissue sections | Yes |
| In vivo neurons | Yes |
| Fixed tissue sections | Yes |
| Cultured cells | Yes |
| Cell loading | Iontophoresis, microinjection, or crystal/paste placement |
| Staining mode | Exogenous |
| Best for | Cell populations |
| Transport | Bidirectional |
| Synaptic transfer | No |
| Gap junction transfer | No |
| Cat. No. | Product Name | Standard filter set(s) | Fluorophore | Ex/Em (nm) | Fixable | Format |
| NTA-2011-ZP120 | "DiI" - DiIC18(3), Solid | Rhodamine | DiI | 549/565 | No | Solid |
| NTA-2011-ZP121 | "DiI" - DiIC18(3), Crystal | Crystal | ||||
| NTA-2011-ZP122 | DiI Cell-Labeling Solution | Solution | ||||
| NTA-2011-ZP123 | NewTrace™ CM-DiI Dye, Solid | CM DiI | 549/565 | Yes | Solid | |
| NTA-2011-ZP48 | NewTrace™ CM-DiI Tissue Labeling Paste | Paste | ||||
| NTA-2011-ZP124 | CM-DiI Cell-Labeling Solution | Solution | ||||
| NTA-2011-ZP125 | "DiD" - DiIC18(5), Solid | Cy®5 | DiD | 644/665 | No | Solid |
| NTA-2011-ZP126 | "DiR" - DiIC18(7), Solid | Cy®5.5 | DiR | 750/780 | Solid |
In addition to reactive dye formulations, we also provide dyes conjugated with a variety of antibodies, peptides, proteins, tracers and amplification substrates to optimize cell labeling and detection. Please contact us for more details.
