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- NeuroMab™ Anti-pTau Antibody,Clone NR3595P (Cat#: NRP-0422-P1719)
- NeuroMab™ Anti-GD2 Antibody,Clone NR3007P (Cat#: NRZP-1222-ZP767)
- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- NeuroMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- NeuroMab™ Anti-Tau Antibody,Clone NR2218P (Cat#: NRP-0422-P2275)
- NeuroMab™ Anti-SEZ6 Antibody, Clone NR28P (Cat#: NRP-0422-P515)
- NeuroMab™ Anti-Amyloid Beta 1-15 Antibody,Clone NR2660P (Cat#: NRP-0422-P867)
- iNeu™ Human Neural Stem Cell Line (Cat#: NCL200552ZP)
- Mouse Microglia N9 (Cat#: NCL2110P073)
- C57 Brain Cortex Neurons [Mouse] (Cat#: NCC20-9PZ48)
- Human Dental Pulp Stem Cells (Cat#: NRZP-1122-ZP113)
- Sf295 Human Glioblastoma Cells (Cat#: NCL-2108P180)
- Human CMEC/D3 Cell Line, Blood-Brain Barrier Model (Cat#: NCL-2108-P020)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Green Fluorescent Alpha-synuclein SH-SY5Y Cell Line (Cat#: NCL2110P209)
- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) (Cat#: NCL-2103-P49)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- AAV2/2Retro-CAG-DIO-EGFP-2A-TetTox-pA [Neural Tracing] (Cat#: NTA-2012-ZP303)
- AAV2/9-hEF1a-DIO-mCherry-P2A-TetTox-WPRE-pA (Cat#: NTA-2012-ZP268)
- pAAV-syn-jGCaMP8f-WPRE (Cat#: NTA-2106-P061)
- pAAV-hSyn-DIO-XCaMP-R-WPRE (Cat#: NTA-2012AD-P508)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- Dextran, Cy5 Labeled, 2000 kDa (Cat#: NRZP-0722-ZP22)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- AAV-mDLX-CRE-tdTomato (Cat#: NRZP-0622-ZP721)
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- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
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- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
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- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
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- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein, HIRMab-NAGLU (Cat#: NRZP-0423-ZP506)
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- NeuroPro™ Anti-EPO BBB Shuttle Protein, HIRMab-EPO (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-ASA BBB Shuttle Protein, HIRMab-ASA (Cat#: NRZP-0423-ZP504)
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- NeuroPro™ Anti-TNFR BBB Shuttle Protein, HIRMab-TNFR (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
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Hydrazide & Biocytin Related Products
Hydrazides and biocytins are a class of cell tracers that can be fixed by aldehyde. These low molecular weight (<1,000 Da) polar tracers cross gap junctions and follow neuronal projections. Biocytin hydrazide provides a variety of fluorescent colors and can be used in multiples with other probes. Biocytins are easy to accept secondary detection for effective signal amplification.
Advantages of hydrazide and biocytin for neuron staining:
- Loading by microinjection or iontophoresis
- Can be fixed and light stable
- Able to connect through gapss
- Use direct detection or signal amplification
Applications
With low molecular weight and bright fluorescent signals, these cell-impermeable polar tracers can be used to track neuronal projections and study gap junctions. They have immobility and light stability, and provide a variety of colors, and can be multiplexed with other fluorescent probes. The fluorescent streptavidin conjugate can also be used to detect the signal signal to detect biotin.
Since they are connected across gaps, biocytosine and hydrazide are often used together with labeled dextran retained in the cell to study neuronal communication.
Hydrazides | Biocytins | |
Readout |
Direct fluorescence imaging of cells
|
Direct fluorescence imaging of cells; biocytins easily accept secondary detection to amplify weak signals |
Tissue sections | Yes | Yes |
In vivo neurons | Yes | Yes |
Fixed tissue sections | No | No |
Cultured cells | Yes | Yes |
Cell loading | Microinjection, iontophoresis | Microinjection, iontophoresis |
Staining mode | Endogenous | Endogenous |
Best for | Single cells | Endogenous |
Fixable | ||
Transport | Bidirectional | Bidirectional |
Photobleaching resistance | ||
Synaptic transfer | No | No |
Gap junction transfer | Yes | Yes |
Cat. No. | Product Name | Standard filter set(s) | Fluorophore | Ex/Em (nm) |
NTA-2011-ZP80 | Alexa Fluor® 350 Hydrazide | DAPI | Alexa Fluor® 350 | 346/442 |
NTA-2011-ZP81 | Alexa Fluor® 488 Hydrazide | FITC | Alexa Fluor® 488 | 495/519 |
NTA-2011-ZP82 | Alexa Fluor® 568 Hydrazide | Rhodamine | Alexa Fluor® 568 | 578/603 |
NTA-2011-ZP83 | Alexa Fluor® 594 Hydrazide | Texas Red® | Alexa Fluor® 594 | 590/617 |
NTA-2011-ZP84 | Alexa Fluor® 555 Hydrazide | TRITC | Alexa Fluor® 555 | 555/565 |
NTA-2011-ZP85 | Alexa Fluor® 633 Hydrazide | Texas Red® | Alexa Fluor® 633 | 632/647 |
NTA-2011-ZP86 | Alexa Fluor® 647 Hydrazide | Cy®5 | Alexa Fluor® 647 | 650/668 |
Cat. No. | Product Name | Standard filter set(s) | Fluorophore | Ex/Em (nm) |
NTA-2011-ZP87 | Alexa Fluor® 488 Biocytin | FITC | Alexa Fluor® 488 | 495/519 |
NTA-2011-ZP89 | Alexa Fluor® 594 Biocytin | Texas Red® | Alexa Fluor® 594 | 590/617 |
In addition to reactive dye formulations, we also provide AlexaFluor dyes conjugated with a variety of antibodies, peptides, proteins, tracers and amplification substrates to optimize cell labeling and detection. Please contact us for more details.