Hydrazides & Biocytins
Hydrazides and biocytins are a class of cell tracers that can be fixed by aldehyde. These low molecular weight (<1,000 Da) polar tracers cross gap junctions and follow neuronal projections. Biocytin hydrazide provides a variety of fluorescent colors and can be used in multiples with other probes. Biocytins are easy to accept secondary detection for effective signal amplification.
Advantages of hydrazide and biocytin for neuron staining：
- Loading by microinjection or iontophoresis
- Can be fixed and light stable
- Able to connect through gapss
- Use direct detection or signal amplification
With low molecular weight and bright fluorescent signals, these cell-impermeable polar tracers can be used to track neuronal projections and study gap junctions. They have immobility and light stability, and provide a variety of colors, and can be multiplexed with other fluorescent probes. The fluorescent streptavidin conjugate can also be used to detect the signal signal to detect biotin.
Since they are connected across gaps, biocytosine and hydrazide are often used together with labeled dextran retained in the cell to study neuronal communication.
In addition to reactive dye formulations, we also provide AlexaFluor dyes conjugated with a variety of antibodies, peptides, proteins, tracers and amplification substrates to optimize cell labeling and detection. Please contact us for more details.